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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BTNL2 Polyclonal antibody (25110-1-AP)

    WB analysis using 25110-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25110-1-AP (BTNL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using BTNL2 Polyclonal antibody (25110-1-AP)

    WB analysis of mouse kidney using 25110-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25110-1-AP (BTNL2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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BTNL2 Polyclonal antibody

BTNL2 Polyclonal Antibody for WB, ELISA
Cat No. 25110-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

BTL II, BTNL2, Butyrophilin like protein 2, HSBLMHC1, SS2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHL-60 cells, mouse kidney tissue, HepG2 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

25110-1-AP targets BTNL2 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BTNL2 fusion protein Ag18803 相同性解析による交差性が予測される生物種
Full Name butyrophilin-like 2 (MHC class II associated)
Calculated molecular weight 455 aa, 50 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC127642
Gene Symbol BTNL2
Gene ID (NCBI) 56244
RRIDAB_2879902
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UIR0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Butyrophilin-like protein 2 (BTNL2) is a transmembrane immunoregulatory protein that is highly expressed in the gastrointestinal tract. BTNL2 belongs to the butyrophilin-like family of proteins, and several of the butyrophilins and butyrophilin-like proteins, such as BTN2A1, BTN3A1, skint-1, BTNL1 and BTNL6, have been shown to play an essential role in regulating γδ T cell development and differentiation.

Protocols

Product Specific Protocols
WB protocol for BTNL2 antibody 25110-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Cancer cell-expressed BTNL2 facilitates tumour immune escape via engagement with IL-17A-producing γδ T cells.

Authors - Yanyun Du
View Article