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  • Featured Product
  • KD/KO Validated

BUB1 Polyclonal antibody, PBS Only

BUB1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, canine

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13330-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of MKN-45 cells using BUB1 Polyclonal antibody (13330-1-AP)

    WB analysis of MKN-45 using 13330-1-AP (same clone as 13330-1-PBS)

    MKN-45 cells were subjected to SDS PAGE followed by western blot with 13330-1-AP (BUB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13330-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using BUB1 Polyclonal antibody (13330-1-AP)

    WB analysis using 13330-1-AP (same clone as 13330-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 13330-1-AP (BUB1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13330-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using BUB1 Polyclonal antibody (13330-1-AP)

    WB analysis of HeLa using 13330-1-AP (same clone as 13330-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 13330-1-AP (BUB1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13330-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SGC-7901 cells using BUB1 Polyclonal antibody (13330-1-AP)

    WB analysis of SGC-7901 using 13330-1-AP (same clone as 13330-1-PBS)

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 13330-1-AP (BUB1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13330-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of IMR-90 cells using BUB1 Polyclonal antibody (13330-1-AP)

    IF Staining of IMR-90 cells using 13330-1-AP (same clone as 13330-1-PBS)

    IF results of BUB1 anitbody with IMR-90 cells by M.S. Grasiella Angelina Andriani Cristina Montagna Lab at Albert Einstein College of Medicine-Bronx - NY. This data was developed using the same antibody clone with 13330-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using BUB1 Polyclonal antibody (13330-1-AP)

    IF Staining of MDCK using 13330-1-AP (same clone as 13330-1-PBS)

    Immunofluorescent analysis of MDCK cells using 13330-1-AP (BUB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13330-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13330-1-PBS targets BUB1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, canine samples.

Tested Reactivity human, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BUB1 fusion protein Ag4138 相同性解析による交差性が予測される生物種
Full Name budding uninhibited by benzimidazoles 1 homolog (yeast)
Calculated molecular weight 1085 aa, 122 kDa
Observed molecular weight 45 kDa, 120 kDa
GenBank accession numberBC028201
Gene symbol BUB1
Gene ID (NCBI) 699
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Bub1 is an evolutionarily conserved mitotic checkpoint control protein that is present in diverse organisms including yeast and humans. Bub1 is a serine/threonine protein kinase and is required for recruitment of Mad1, Mad2, Bub3, and CENP-E to kinetochores. Full-length Bub1 protein was cleaved to an 45 kDa fragment and an intermediate fragment of 90 kDa, only detected at early stages of apoptosis execution, indicating cleavage of Bub1 in the amino- and carboxy-terminal regions of the protein(PMID:15818396).