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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BUB1B Monoclonal antibody

BUB1B Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3B2D5

Cat no : 60423-1-Ig

Synonyms

BubR1, hBUBR1, EC:2.7.11.1, BUB1beta, Bub1A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BUB1B Monoclonal antibody (60423-1-Ig)

    WB analysis using 60423-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60423-1-Ig (BUB1B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using BUB1B Monoclonal antibody (60423-1-Ig)

    WB analysis using 60423-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60423-1-Ig (BUB1B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHaCaT cells, Saos-2 cells, U2OS cells, HeLa cells, HEK-293 cells, Ramos cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60423-1-Ig targets BUB1B in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen BUB1B fusion protein Ag34182 相同性解析による交差性が予測される生物種
Full Name budding uninhibited by benzimidazoles 1 homolog beta (yeast)
Calculated molecular weight 1050 aa, 120 kDa
Observed molecular weight120-130 kDa
GenBank accession numberBC018739
Gene symbol BubR1
Gene ID (NCBI) 701
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BUB1B(Mitotic checkpoint serine/threonine-protein kinase BUB1 beta), also named BUBR1, MAD3Land SSK1, belongs to the BUB1 subfamily. Human BUB1B is a 120 kDa protein that plays a central role in the spindle assembly checkpoint (SAC), the evolutionary conserved and self-regulatory system of higher organisms that monitors and repairs defects in chromosome segregation in mitotic cells(PMID:22201036). In addition to phosphorylation, BUB1B is also subjected to posttranslational modifications including acetylation(PMID:22167194). Defects in BUB1B are the cause of premature chromatid separation trait (PCS) and mosaic variegated aneuploidy syndrome type 1 (MVA1).

Protocols

Product Specific Protocols
WB protocol for BUB1B antibody 60423-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols