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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BUB1B Recombinant antibody

BUB1B Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240969F7

Cat no : 83920-6-RR

Synonyms

BubR1, hBUBR1, EC:2.7.11.1, BUB1beta, Bub1A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BUB1B Recombinant antibody (83920-6-RR)

    WB analysis using 83920-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83920-6-RR (BUB1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using BUB1B Recombinant antibody (83920-6-RR)

    IHC staining of mouse lung using 83920-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83920-6-RR (BUB1B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BUB1B Recombinant antibody (83920-6-RR)

    IF Staining of HeLa using 83920-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BUB1B antibody (83920-6-RR, Clone: 240969F7 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83920-6-RR

    Affinity and Kinetic Characterization of 83920-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83920-6-RR against Human BUB1B were performed. The affinity constant is 7.60 nM.

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Tested Applications

Positive WB detected inHeLa cells, Raji cells
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83920-6-RR is BUB1B Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen BUB1B fusion protein Ag34182 相同性解析による交差性が予測される生物種
Full Name budding uninhibited by benzimidazoles 1 homolog beta (yeast)
Calculated molecular weight 1050 aa, 120 kDa
Observed molecular weight130 kDa
GenBank accession numberBC018739
Gene symbol BubR1
Gene ID (NCBI) 701
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BUB1B(Mitotic checkpoint serine/threonine-protein kinase BUB1 beta), also named BUBR1, MAD3Land SSK1, belongs to the BUB1 subfamily. Human BUB1B is a 120 kDa protein that plays a central role in the spindle assembly checkpoint (SAC), the evolutionary conserved and self-regulatory system of higher organisms that monitors and repairs defects in chromosome segregation in mitotic cells(PMID:22201036). In addition to phosphorylation, BUB1B is subjected to posttranslational modifications including acetylation(PMID:22167194). Defects in BUB1B cause premature chromatid separation trait (PCS) and mosaic variegated aneuploidy syndrome type 1 (MVA1).

Protocols

Product Specific Protocols
WB protocol for BUB1B antibody 83920-6-RRDownload protocol
IHC protocol for BUB1B antibody 83920-6-RRDownload protocol
IF protocol for BUB1B antibody 83920-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols