BUB1B Recombinant antibody
BUB1B Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
240969F7
Cat no : 83920-6-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, Raji cells |
Positive IHC detected in | mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of 83920-6-RR is BUB1B Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | BUB1B fusion protein Ag34182 相同性解析による交差性が予測される生物種 |
Full Name | budding uninhibited by benzimidazoles 1 homolog beta (yeast) |
Calculated molecular weight | 1050 aa, 120 kDa |
Observed molecular weight | 130 kDa |
GenBank accession number | BC018739 |
Gene symbol | BubR1 |
Gene ID (NCBI) | 701 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BUB1B(Mitotic checkpoint serine/threonine-protein kinase BUB1 beta), also named BUBR1, MAD3Land SSK1, belongs to the BUB1 subfamily. Human BUB1B is a 120 kDa protein that plays a central role in the spindle assembly checkpoint (SAC), the evolutionary conserved and self-regulatory system of higher organisms that monitors and repairs defects in chromosome segregation in mitotic cells(PMID:22201036). In addition to phosphorylation, BUB1B is subjected to posttranslational modifications including acetylation(PMID:22167194). Defects in BUB1B cause premature chromatid separation trait (PCS) and mosaic variegated aneuploidy syndrome type 1 (MVA1).
Protocols
Product Specific Protocols | |
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WB protocol for BUB1B antibody 83920-6-RR | Download protocol |
IHC protocol for BUB1B antibody 83920-6-RR | Download protocol |
IF protocol for BUB1B antibody 83920-6-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |