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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BUB3 Recombinant antibody

BUB3 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240087D9

Cat no : 83266-5-RR

Synonyms

hBUB3, BUB3L, BUB 3, 240087D9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BUB3 Recombinant antibody (83266-5-RR)

    WB analysis using 83266-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83266-5-RR (BUB3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BUB3 Recombinant antibody (83266-5-RR)

    IF Staining of HeLa using 83266-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BUB3 antibody (83266-5-RR, Clone: 240087D9 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BUB3 Recombinant antibody (83266-5-RR)

    IF Staining of HeLa using 83266-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BUB3 antibody (83266-5-RR, Clone: 240087D9 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using BUB3 Recombinant antibody (83266-5-RR)

    FC experiment of HeLa using 83266-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug BUB3 Recombinant antibody (83266-5-RR, Clone:240087D9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83266-5-RR

    Affinity and Kinetic Characterization of 83266-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83266-5-RR against Human BUB3 were performed. The affinity constant is 0.228 nM.

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Tested Applications

Positive WB detected inHeLa cells, HEK-293T cells, MCF-7 cells, Jurkat cells, C6 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83266-5-RR is BUB3 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen BUB3 fusion protein Ag25608 相同性解析による交差性が予測される生物種
Full Name budding uninhibited by benzimidazoles 3 homolog (yeast)
Calculated molecular weight 37 kDa
Observed molecular weight 37-40 kDa
GenBank accession numberBC005138
Gene symbol BUB3
Gene ID (NCBI) 9184
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for BUB3 antibody 83266-5-RRDownload protocol
IF protocol for BUB3 antibody 83266-5-RRDownload protocol
FC protocol for BUB3 antibody 83266-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols