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KD/KO Validated

BUD31 Polyclonal antibody

Name: BUD31 Polyclonal antibody
Brand: Proteintech
SKU: 11798-1-AP

BUD31 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, RIP, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11798-1-AP

Synonyms

BUD31, BUD31 homolog (S. cerevisiae), EDG 2, EDG2, fSAP17, G10, Protein BUD31 homolog, Protein EDG 2, Protein G10 homolog, YCR063W

Validation Data Gallery

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AllWBIPIHC

WB analysis of mouse brain using 11798-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 11798-1-AP (BUD31 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 11798-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 11798-1-AP (BUD31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 11798-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 11798-1-AP (BUD31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 11798-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 11798-1-AP (BUD31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 11798-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 11798-1-AP (BUD31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 11798-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 11798-1-AP (BUD31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11798-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11798-1-AP (BUD31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of hESC using 11798-1-AP
hESC cells were subjected to SDS PAGE followed by western blot with 11798-1-AP (BUD31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 11798-1-AP
IP result of anti-BUD31 (IP:11798-1-AP, 4ug; Detection:11798-1-AP 1:1500) with mouse brain tissue lysate 3440ug.
IHC staining of human colon cancer using 11798-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 11798-1-AP (BUD31 antibody) at dilution of 1:100 (under 10x lens).

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Cat No. 11798-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse brain tissue, hESC cells, human brain tissue, Jurkat cells, mouse heart tissue, mouse liver tissue, mouse lung tissue, Raji cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 3 publications below
IHC	See 1 publications below
IF	See 1 publications below
CoIP	See 1 publications below
RIP	See 2 publications below

Product Information

11798-1-AP targets BUD31 in WB, IP, IF, RIP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	BUD31 fusion protein Ag2373 相同性解析による交差性が予測される生物種
Full Name	BUD31 homolog (S. cerevisiae)
Calculated molecular weight	144 aa, 17 kDa
Observed molecular weight	17-20 kDa
GenBank accession number	BC022821
Gene symbol	BUD31
Gene ID (NCBI)	8896
RRID	AB_2274894
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for BUD31 antibody 11798-1-AP	Download protocol
IHC protocol for BUD31 antibody 11798-1-AP	Download protocol
IP protocol for BUD31 antibody 11798-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature The spliceosome is a therapeutic vulnerability in MYC-driven cancer. Authors - Tiffany Y-T Hsu KD Validated View Article
human	WB	iScience Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells. Authors - Mio Iwasaki View Article
mouse	WB,RIP	Cell Death Differ Bud31-mediated alternative splicing is required for spermatogonial stem cell self-renewal and differentiation Authors - Junchao Qin KD Validated View Article
human	IHC,IF,CoIP,RIP	Nat Commun Splicing factor BUD31 promotes ovarian cancer progression through sustaining the expression of anti-apoptotic BCL2L12 Authors - Zixiang Wang KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BUD31 Polyclonal antibody

BUD31 Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis of mouse brain using 11798-1-AP

WB analysis of Jurkat using 11798-1-AP

WB analysis of mouse heart using 11798-1-AP

WB analysis of Raji using 11798-1-AP

WB analysis of mouse lung using 11798-1-AP

WB analysis of mouse liver using 11798-1-AP

WB analysis of human brain using 11798-1-AP

WB analysis of hESC using 11798-1-AP

IP experiment of mouse brain using 11798-1-AP

IHC staining of human colon cancer using 11798-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

The spliceosome is a therapeutic vulnerability in MYC-driven cancer.

Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.

Bud31-mediated alternative splicing is required for spermatogonial stem cell self-renewal and differentiation

Splicing factor BUD31 promotes ovarian cancer progression through sustaining the expression of anti-apoptotic BCL2L12