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Validation Data Gallery

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  • Western Blot (WB) analysis of Neuro-2a cells using BZRAP1 Polyclonal antibody (28241-1-AP)

    WB analysis of Neuro-2a using 28241-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 28241-1-AP (BZRAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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BZRAP1 Polyclonal antibody

BZRAP1 Polyclonal Antibody for WB, ELISA
Cat No. 28241-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Synonyms

BZRAP1, DKFZp686F02123, KIAA0612, PBR IP, PRAX 1, PRAX1, RBP1, RIM BP1, RIMBP1, RIMS binding protein 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNeuro-2a cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28241-1-AP targets BZRAP1 in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BZRAP1 fusion protein Ag28198 相同性解析による交差性が予測される生物種
Full Name benzodiazapine receptor (peripheral) associated protein 1
Calculated molecular weight 1857 aa, 200 kDa
Observed molecular weight 200-235 kDa
GenBank accession numberBC146852
Gene Symbol BZRAP1
Gene ID (NCBI) 9256
RRIDAB_2881096
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95153
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BZRAP1, also named as TSPOAP1 and PBR-associated protein 1 (PRAX-1), is a cytoplasmic protein of 1857 residues. It plays a key role in inflammation, neuroinflammation, mental and metabolic diseases, and cancer. BZRAP1 predominantly expressed in the central nervous system, pituitary gland, and thymus, specially abundant in the CA1 subfield of the hippocampus. Tumor necrosis factor receptor 1 (TNFR1), vascular cell adhesion molecule 1 (VCAM1), cyclic AMP response element-binding protein 1 (CREB1), T-cell receptor (TCR), nucleotide-binding oligomerization domain, leucine-rich repeat and pyrin domain containing 3 (NLRP3), DNA-dependent protein kinase (DNA-PK or PRKDC), and mitochondrial permeability transition pore (mPTP) were the major interaction partners of BZRAP1 (PMID: 34212002).

Protocols

Product Specific Protocols
WB protocol for BZRAP1 antibody 28241-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols