Validation Data Gallery

Filter: AllWBIHCIF/ICCFC (Intra)Cytometric bead array

WB analysis of Jurkat using 66020-1-Ig (same clone as 66020-1-PBS)
Jurkat, Ramos, K-562 and Raw 264.7 cells were subjected to SDS PAGE followed by western blot with 66020-1-Ig (Bcl-xL Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.
WB analysis of A431 using 66020-1-Ig (same clone as 66020-1-PBS)
A431 cells were subjected to SDS PAGE followed by western blot with 66020-1-Ig (Bcl-xL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 66020-1-Ig (same clone as 66020-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 66020-1-Ig (Bcl-xL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 66020-1-Ig (same clone as 66020-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 66020-1-Ig (Bcl-xL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 66020-1-Ig (same clone as 66020-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66020-1-Ig (Bcl-XL antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 66020-1-Ig (same clone as 66020-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66020-1-Ig (Bcl-XL antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.
IHC staining of human lymphoma using 66020-1-Ig (same clone as 66020-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66020-1-Ig (Bcl-XL antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 66020-1-Ig (same clone as 66020-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon using 66020-1-Ig(Bcl-xL antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 66020-1-Ig (same clone as 66020-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon using 66020-1-Ig(Bcl-xL antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 66020-1-Ig (same clone as 66020-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 66020-1-Ig(Bcl-xL antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 66020-1-Ig (same clone as 66020-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 66020-1-Ig(Bcl-xL antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.
IHC staining of human lymphoma using 66020-1-Ig (same clone as 66020-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66020-1-Ig (Bcl-XL antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 66020-1-Ig (same clone as 66020-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Bcl-XL antibody (66020-1-Ig, Clone: 4C12A6 ) at dilution of 1:1355 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 66020-1-Ig (same clone as 66020-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Bcl-XL antibody (66020-1-Ig, Clone: 4C12A6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.
FC experiment of HeLa using 66020-1-Ig (same clone as 66020-1-PBS)
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Bcl-XL (66020-1-Ig, Clone:4C12A6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.
×
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Bcl-XL (66020-1-Ig, Clone:4C12A6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.
Cytometric bead array standard curve of MP50613-1
Cytometric bead array standard curve of MP50613-1, Bcl-xL Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66020-2-PBS. Detection antibody: 66020-1-PBS. Standard:Ag18037. Range: 1.563-100 ng/mL.

Bcl-XL Monoclonal antibody, PBS Only (Detector)

Bcl-XL Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 66020-1-PBS

Clone No.4C12A6

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

BCL2L1, Bcl-2-like protein 1, Bcl2-L-1, Apoptosis regulator Bcl-X, Apoptosis regulator Bcl X

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66020-1-PBS targets Bcl-XL as part of a matched antibody pair:

MP50613-1: 66020-2-PBS capture and 66020-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	Bcl-XL fusion protein Ag18037 相同性解析による交差性が予測される生物種
Full Name	BCL2-like 1
Calculated molecular weight	26 kDa
Observed molecular weight	30 kDa
GenBank accession number	BC019307
Gene Symbol	Bcl-XL
Gene ID (NCBI)	598
RRID	AB_11042315
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q07817
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Jurkat using 66020-1-Ig (same clone as 66020-1-PBS)

WB analysis of A431 using 66020-1-Ig (same clone as 66020-1-PBS)

WB analysis of HeLa using 66020-1-Ig (same clone as 66020-1-PBS)

WB analysis of Jurkat using 66020-1-Ig (same clone as 66020-1-PBS)

IHC staining of human breast cancer using 66020-1-Ig (same clone as 66020-1-PBS)

IHC staining of human breast cancer using 66020-1-Ig (same clone as 66020-1-PBS)

IHC staining of human lymphoma using 66020-1-Ig (same clone as 66020-1-PBS)

IHC staining of human colon using 66020-1-Ig (same clone as 66020-1-PBS)

IHC staining of human colon using 66020-1-Ig (same clone as 66020-1-PBS)

IHC staining of human testis using 66020-1-Ig (same clone as 66020-1-PBS)

IHC staining of human testis using 66020-1-Ig (same clone as 66020-1-PBS)

IHC staining of human lymphoma using 66020-1-Ig (same clone as 66020-1-PBS)

IF Staining of HepG2 using 66020-1-Ig (same clone as 66020-1-PBS)

IF Staining of HeLa using 66020-1-Ig (same clone as 66020-1-PBS)

FC experiment of HeLa using 66020-1-Ig (same clone as 66020-1-PBS)

Cytometric bead array standard curve of MP50613-1