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KD/KO Validated

Bcl-XL Polyclonal antibody

Name: Bcl-XL Polyclonal antibody
Brand: Proteintech
SKU: 10783-1-AP
Rating: 3.50 (2 reviews)

Bcl-XL Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

186

Reviews (2)

Cat no : 10783-1-AP

Synonyms

BCL2L1, Bcl-2-like protein 1, Bcl2-L-1, Apoptosis regulator Bcl-X, Apoptosis regulator Bcl X

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 10783-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa cells using 10783-1-AP
WB result of Bcl-XL antibody (10783-1-AP, 1:800) with si-Control and si-Bcl-XL transfected HeLa cells.
WB analysis of C6 using 10783-1-AP
C6 cells were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse placenta using 10783-1-AP
mouse placenta tissue were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of RAW264.7 using 10783-1-AP
RAW264.7 cells were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 10783-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:250 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10783-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 10783-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 10783-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 10783-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 10783-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 10783-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of k-562 using 10783-1-AP
IP result of anti-Bcl-XL (IP:10783-1-AP, 4ug; Detection:10783-1-AP 1:1000) with k-562 cells lysate 1760 ug.
IHC staining of human lung cancer using 10783-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10783-1-AP (Bcl-XL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 10783-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10783-1-AP (Bcl-XL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 10783-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10783-1-AP (Bcl-XL antibody at dilution of 1:200 (under 10x lens).
IHC staining of human colon using 10783-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10783-1-AP (Bcl-XL antibody at dilution of 1:200 (under 40x lens).
IF Staining of NIH/3T3 using 10783-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Bcl-XL antibody (10783-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Cat No. 10783-1-AP

Size/Concentration

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A549 cells, Raji cells, Jurkat cells, rat brain tissue, HeLa cells, NIH/3T3 cells, human placenta tissue, C6 cells, K-562 cells, mouse placenta tissue, RAW264.7
Positive IP detected in	k-562 cells
Positive IHC detected in	human lung cancer tissue, human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	NIH/3T3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 173 publications below
IHC	See 9 publications below
IF	See 2 publications below
IP	See 1 publications below
ChIP	See 1 publications below

Product Information

10783-1-AP targets Bcl-XL in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, zebrafish, hamster, sheep, goat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Bcl-XL fusion protein Ag1232 相同性解析による交差性が予測される生物種
Full Name	BCL2-like 1
Calculated molecular weight	26 kDa
Observed molecular weight	30 kDa
GenBank accession number	BC019307
Gene symbol	Bcl-XL
Gene ID (NCBI)	598
RRID	AB_2064724
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L), Bcl-X(s), and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-X(L) isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can form heterodimers with BAX, BAK, or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-X(s) isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator. Bcl-X(beta) is a 227 amino acid protein. This antibody can recognize Bcl-XL, Bcl-X(s) and Bcl-X(Beta).

Protocols

Product Specific Protocols
WB protocol for Bcl-XL antibody 10783-1-AP	Download protocol
IHC protocol for Bcl-XL antibody 10783-1-AP	Download protocol
IF protocol for Bcl-XL antibody 10783-1-AP	Download protocol
IP protocol for Bcl-XL antibody 10783-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
		Immunity cis-B7:CD28 interactions at invaginated synaptic membranes provide CD28 co-stimulation and promote CD8+ T cell function and anti-tumor immunity Authors - Yunlong Zhao View Article
human	WB	Nat Commun Structural and functional analyses of hepatitis B virus X protein BH3-like domain and Bcl-xL interaction. Authors - Tian-Ying Zhang View Article
human	WB	Carbohydr Polym A multi-bioresponsive self-assembled nano drug delivery system based on hyaluronic acid and geraniol against liver cancer Authors - Shaofeng Duan View Article
mouse	WB	Adv Healthc Mater Copper-Based Composites Nanoparticles Improve Triple-Negative Breast Cancer Treatment with Induction of Apoptosis-Cuproptosis and Immune Activation Authors - Ning Wang View Article
human,mouse	WB	Cancer Res Bcl-xL enforces a slow-cycling state necessary for survival in the nutrient-deprived microenvironment of pancreatic cancer. Authors - Yogev Sela View Article
rat	WB	Phytomedicine LC-MS based metabonomics study on protective mechanism of ESWW in cerebral ischemia via CYTC/Apaf-1/NDRG4 pathway Authors - Yan Liang View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Shinford (Verified Customer) (10-26-2023)

In cancer cell lines, the bands appeared significantly clearer, and the optimal concentration was found to be 1:1000.

Applications: Western Blot
Primary Antibody Dilution: 1:1000
Cell Tissue Type: Lung cancer cell lines

Bcl-XL Antibody Western Blot validation (1:1000 dilution) in Lung cancer cell lines (Cat no:10783-1-AP)

Bastien (Verified Customer) (08-19-2020)

Staining of B cells from mice bone marrow after cytoplasmic permezbiliation. The cells were first fixed and permeabilized with intracellular fix and perm set from ebioscience and then stained with 0,25 µl/ million cells with PiP5Ka antibody (15713-1-AP) during 50min After, a seconde staining was performed with 0,05µl/million cells of F(ab')2-Donkey anti-Rabbit IgG (H+L), PE, Secondary Antibody from invitrogen. In blue secondary antibody alone and in red primary (10783-1-AP) +secondary antibody

Applications: Flow Cytometry
Primary Antibody Dilution: 0,05µl/10^6 cells
Cell Tissue Type: B cells

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Bcl-XL Polyclonal antibody

Bcl-XL Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 10783-1-AP

WB analysis of HeLa cells using 10783-1-AP

WB analysis of C6 using 10783-1-AP

WB analysis of mouse placenta using 10783-1-AP

WB analysis of RAW264.7 using 10783-1-AP

WB analysis of human placenta using 10783-1-AP

WB analysis of HepG2 using 10783-1-AP

WB analysis of Raji using 10783-1-AP

WB analysis of K-562 using 10783-1-AP

WB analysis of NIH/3T3 using 10783-1-AP

WB analysis of Jurkat using 10783-1-AP

WB analysis of rat brain using 10783-1-AP

IP experiment of k-562 using 10783-1-AP

IHC staining of human lung cancer using 10783-1-AP

IHC staining of human lung cancer using 10783-1-AP

IHC staining of human colon using 10783-1-AP

IHC staining of human colon using 10783-1-AP

IF Staining of NIH/3T3 using 10783-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

cis-B7:CD28 interactions at invaginated synaptic membranes provide CD28 co-stimulation and promote CD8+ T cell function and anti-tumor immunity

Structural and functional analyses of hepatitis B virus X protein BH3-like domain and Bcl-xL interaction.

A multi-bioresponsive self-assembled nano drug delivery system based on hyaluronic acid and geraniol against liver cancer

Copper-Based Composites Nanoparticles Improve Triple-Negative Breast Cancer Treatment with Induction of Apoptosis-Cuproptosis and Immune Activation

Bcl-xL enforces a slow-cycling state necessary for survival in the nutrient-deprived microenvironment of pancreatic cancer.

LC-MS based metabonomics study on protective mechanism of ESWW in cerebral ischemia via CYTC/Apaf-1/NDRG4 pathway

Reviews

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