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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Bcl-xL Recombinant antibody

Bcl-xL Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240450A9

Cat no : 83330-2-RR

Synonyms

Apoptosis regulator Bcl X, BCL2L1, DKFZp781P2092

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Bcl-xL Recombinant antibody (83330-2-RR)

    IF Staining of HeLa using 83330-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Bcl-xL antibody (83330-2-RR, Clone: 240450A9 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Flow cytometry (FC) experiment of Jurkat cells using Bcl-xL Recombinant antibody (83330-2-RR)

    FC experiment of Jurkat using 83330-2-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug Bcl-xL Recombinant antibody (83330-2-RR, Clone:240450A9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of HeLa cells using Bcl-xL Recombinant antibody (83330-2-RR)

    FC experiment of HeLa using 83330-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Bcl-xL Recombinant antibody (83330-2-RR, Clone:240450A9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells, Jurkat cells
Positive FC detected inHeLa cells, Jurkat cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83330-2-RR is Bcl-xL Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Bcl-xL fusion protein Ag25604 相同性解析による交差性が予測される生物種
Full Name BCL2-like 1
Calculated molecular weight 26 kDa
GenBank accession numberBC019307
Gene symbol Bcl-XL
Gene ID (NCBI) 598
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L), Bcl-X(s), and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-X(L) isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can form heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-X(s) isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator. Bcl-X(beta) is a 227 amino acid protein.

Protocols

Product Specific Protocols
IF protocol for Bcl-xL antibody 83330-2-RRDownload protocol
FC protocol for Bcl-xL antibody 83330-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols