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Bcl2 Polyclonal antibody

Name: Bcl2 Polyclonal antibody
Brand: Proteintech
SKU: 26593-1-AP
Rating: 4.67 (3 reviews)

Bcl2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat and More (6)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

846

Reviews (3)

Cat no : 26593-1-AP

Synonyms

Bcl-2, Bcl 2, B cell leukemia/lymphoma 2

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 26593-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 26593-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 26593-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 26593-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 26593-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 26593-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of C2C12 using 26593-1-AP
C2C12 cells were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of mouse spleen using 26593-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26593-1-AP (Bcl2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 26593-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26593-1-AP (Bcl2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse spleen using 26593-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26593-1-AP (Bcl2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 26593-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26593-1-AP (Bcl2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse spleen using 26593-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26593-1-AP (Bcl2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of NIH/3T3 using 26593-1-AP
Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Bcl2 antibody (26593-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of NIH/3T3 using 26593-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Bcl2 antibody (26593-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of NIH/3T3 using 26593-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using 26593-1-AP (Bcl2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 26593-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	NIH/3T3 cells, C2C12 cells, mouse brain tissue, mouse heart tissue, mouse testis tissue, mouse kidney tissue, mouse spleen tissue, rat spleen tissue, rat kidney tissue, rat testis tissue, mouse colon tissue
Positive IHC detected in	mouse spleen tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	NIH/3T3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 813 publications below
IHC	See 3 publications below
IF	See 18 publications below
CoIP	See 1 publications below

Product Information

26593-1-AP targets Bcl2 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity	mouse, rat
Cited Reactivity	mouse, rat, pig, canine, chicken, bovine, hamster, duck
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Bcl2 fusion protein Ag24260 相同性解析による交差性が予測される生物種
Full Name	B-cell leukemia/lymphoma 2
Calculated molecular weight	26 kDa
Observed molecular weight	26 kDa
GenBank accession number	NM-009741
Gene symbol	Bcl2
Gene ID (NCBI)	12043
RRID	AB_2818996
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

1. What is Bcl-2?

Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.

2. FAQs for Bcl-2

How to measure Bcl-2 and Bax apoptotic markers by western blotting
Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.
What band size should I expect in western blotting for Bcl-2?
The molecular weight of Bcl-2 is 26 kDa.

Protocols

Product Specific Protocols
WB protocol for Bcl2 antibody 26593-1-AP	Download protocol
IHC protocol for Bcl2 antibody 26593-1-AP	Download protocol
IF protocol for Bcl2 antibody 26593-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
		ACS Nano Melatonin-Derived Carbon Dots with Free Radical Scavenging Property for Effective Periodontitis Treatment via the Nrf2/HO-1 Pathway Authors - Xirui Xin View Article
mouse	WB	Acta Pharm Sin B Honokiol alleviated neurodegeneration by reducing oxidative stress and improving mitochondrial function in mutant SOD1 cellular and mouse models of amyotrophic lateral sclerosis Authors - Yujun Zhou View Article
mouse	WB	Redox Biol FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response Authors - Zhe Wang View Article
mouse	WB	J Nanobiotechnology PD-L1 blockade TAM-dependently potentiates mild photothermal therapy against triple-negative breast cancer Authors - Chao Wang View Article
mouse	WB	Adv Healthc Mater GDNF-Loaded Polydopamine Nanoparticles-Based Anisotropic Scaffolds Promote Spinal Cord Repair by Modulating Inhibitory Microenvironment Authors - Jinjin Ma View Article
	WB	J Colloid Interface Sci DNA Damage-Sensitized metal phenolic nanosynergists potentiate Low-Power phototherapy for osteosarcoma therapy Authors - Chunxue Song View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH Charlotte (Verified Customer) (08-15-2024)	The fainter band at the bottom of the membrane corresponds to the expected MW. Done in milk Applications: Western Blot Primary Antibody Dilution: 1/1000 Cell Tissue Type: NIH-3T3 Bcl2 Antibody Western Blot validation (1/1000 dilution) in NIH-3T3 (Cat no:26593-1-AP)
FH Anna (Verified Customer) (10-04-2022)	Promising staining. I have a problem with attaching a photo file. Please check the file below. https://drive.google.com/file/d/15d3t6thx7rcWyN-_DkkKCtpAMyi8jxHO/view Applications: Immunofluorescence Primary Antibody Dilution: 500 Cell Tissue Type: brain frozen sections - mouse
FH PRADEEP (Verified Customer) (11-03-2020)	Giving very good result for IF. Applications: Immunofluorescence Primary Antibody Dilution: 1:100 Cell Tissue Type: Mouse muscle

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Bcl2 Polyclonal antibody

Bcl2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 26593-1-AP

WB analysis using 26593-1-AP

WB analysis using 26593-1-AP

WB analysis using 26593-1-AP

WB analysis of mouse testis using 26593-1-AP

WB analysis of mouse heart using 26593-1-AP

WB analysis of C2C12 using 26593-1-AP

IHC staining of mouse spleen using 26593-1-AP

IHC staining of mouse brain using 26593-1-AP

IHC staining of mouse spleen using 26593-1-AP

IHC staining of mouse brain using 26593-1-AP

IHC staining of mouse spleen using 26593-1-AP

IF Staining of NIH/3T3 using 26593-1-AP

IF Staining of NIH/3T3 using 26593-1-AP

IF Staining of NIH/3T3 using 26593-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Melatonin-Derived Carbon Dots with Free Radical Scavenging Property for Effective Periodontitis Treatment via the Nrf2/HO-1 Pathway

Honokiol alleviated neurodegeneration by reducing oxidative stress and improving mitochondrial function in mutant SOD1 cellular and mouse models of amyotrophic lateral sclerosis

FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response

PD-L1 blockade TAM-dependently potentiates mild photothermal therapy against triple-negative breast cancer

GDNF-Loaded Polydopamine Nanoparticles-Based Anisotropic Scaffolds Promote Spinal Cord Repair by Modulating Inhibitory Microenvironment

DNA Damage-Sensitized metal phenolic nanosynergists potentiate Low-Power phototherapy for osteosarcoma therapy

Reviews