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  • Featured Product
  • KD/KO Validated

Mouse Bcl2 Monoclonal antibody, PBS Only

Bcl2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B3F7

Cat no : 68103-1-PBS

Synonyms

AW986256, B cell leukemia/lymphoma 2, Bcl2, Bcl-2, C430015F12Rik, D630044D05Rik, D830018M01Rik

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Bcl2 Monoclonal antibody (68103-1-Ig)

    WB analysis using 68103-1-Ig (same clone as 68103-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68103-1-Ig (Bcl2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68103-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using Bcl2 Monoclonal antibody (68103-1-Ig)

    WB analysis of mouse brain using 68103-1-Ig (same clone as 68103-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 68103-1-Ig (Bcl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68103-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of C2C12 cells using Bcl2 Monoclonal antibody (68103-1-Ig)

    WB analysis of C2C12 using 68103-1-Ig (same clone as 68103-1-PBS)

    C2C12 cells were subjected to SDS PAGE followed by western blot with 68103-1-Ig (Bcl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68103-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Bcl2 Monoclonal antibody (68103-1-Ig)

    WB analysis using 68103-1-Ig (same clone as 68103-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68103-1-Ig (Bcl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68103-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse spleen tissue using Bcl2 Monoclonal antibody (68103-1-Ig)

    WB analysis of mouse spleen using 68103-1-Ig (same clone as 68103-1-PBS)

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 68103-1-Ig (Bcl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68103-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using Bcl2 Monoclonal antibody (68103-1-Ig)

    IHC staining of mouse kidney using 68103-1-Ig (same clone as 68103-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68103-1-Ig (Bcl2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68103-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using Bcl2 Monoclonal antibody (68103-1-Ig)

    IHC staining of mouse kidney using 68103-1-Ig (same clone as 68103-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68103-1-Ig (Bcl2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68103-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat skin tissue using Bcl2 Monoclonal antibody (68103-1-Ig)

    IHC staining of rat skin using 68103-1-Ig (same clone as 68103-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 68103-1-Ig (Bcl2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68103-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat skin tissue using Bcl2 Monoclonal antibody (68103-1-Ig)

    IHC staining of rat skin using 68103-1-Ig (same clone as 68103-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 68103-1-Ig (Bcl2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68103-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using Bcl2 Monoclonal antibody (68103-1-Ig)

    IF Staining of NIH/3T3 using 68103-1-Ig (same clone as 68103-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Bcl2 antibody (68103-1-Ig, Clone: 1B3F7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68103-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using Bcl2 Monoclonal antibody (68103-1-Ig)

    IF Staining of NIH/3T3 using 68103-1-Ig (same clone as 68103-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Bcl2 antibody (68103-1-Ig, Clone: 1B3F7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 68103-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68103-1-PBS targets Bcl2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Bcl2 fusion protein Ag24261 相同性解析による交差性が予測される生物種
Full Name B-cell leukemia/lymphoma 2
Calculated molecular weight 26 kDa
Observed molecular weight26 kDa
GenBank accession numberNM-009741
Gene symbol Bcl2
Gene ID (NCBI) 12043
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Bcl2 is a member of the B cell lymphoma 2 protein family. Members of this family regulate cell death in multiple cell types and can have either proapoptotic or antiapoptotic activities. The protein encoded by this gene inhibits mitochondrial-mediated apoptosis. This protein is an integral outer mitochondrial membrane protein that functions as part of signaling pathway that controls mitochondrial permeability in response to apoptotic stimuli. This protein may also play a role in neuron cell survival and autophagy. Abnormal expression and chromosomal translocations of this gene are associated with cancer progression in numerous tissues.