Bcl2 Recombinant antibody

Bcl2 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240571H5

Cat no : 82469-6-RR

Synonyms

Bcl-2, Bcl 2, B cell leukemia/lymphoma 2, 240571H5


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Tested Applications

Positive WB detected inmouse kidney tissue, mouse spleen tissue, mouse heart tissue
Positive FC (Intra) detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 82469-6-RR is Bcl2 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Bcl2 fusion protein Ag24260 相同性解析による交差性が予測される生物種
Full Name B-cell leukemia/lymphoma 2
Calculated molecular weight 26 kDa
Observed molecular weight26 kDa
GenBank accession numberNM-009741
Gene symbol Bcl2
Gene ID (NCBI) 12043
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Bcl2 is a member of the B cell lymphoma 2 protein family. Members of this family regulate cell death in multiple cell types and can have either proapoptotic or antiapoptotic activities. The protein encoded by this gene inhibits mitochondrial-mediated apoptosis. This protein is an integral outer mitochondrial membrane protein that functions as part of signaling pathway that controls mitochondrial permeability in response to apoptotic stimuli. This protein may also play a role in neuron cell survival and autophagy. Abnormal expression and chromosomal translocations of this gene are associated with cancer progression in numerous tissues.

Protocols

Product Specific Protocols
WB protocol for Bcl2 antibody 82469-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols