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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Beta-2-Microglobulin Polyclonal antibody

Beta-2-Microglobulin Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30226-1-AP

Synonyms

B2M, beta 2 microglobulin, HDCMA22P

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Beta-2-Microglobulin Polyclonal antibody (30226-1-AP)

    WB analysis using 30226-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30226-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Beta-2-Microglobulin Polyclonal antibody (30226-1-AP)

    WB analysis using 30226-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30226-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of NCCIT cells using Beta-2-Microglobulin Polyclonal antibody (30226-1-AP)

    IF Staining of NCCIT using 30226-1-AP

    Immunofluorescent analysis of (4% PFA) fixed NCCIT cells using Beta-2-Microglobulin antibody (30226-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inA431 cells, HepG2 cells, HeLa cells, U-937 cells, Jurkat cells, Raji cells
Positive IF/ICC detected inNCCIT cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30226-1-AP targets Beta-2-Microglobulin in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen fusion protein 相同性解析による交差性が予測される生物種
Full Name beta-2-microglobulin
Calculated molecular weight 119 aa, 14 kDa
Observed molecular weight12-14 kDa
GenBank accession numberBC032589
Gene symbol B2M
Gene ID (NCBI) 567
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.

Protocols

Product Specific Protocols
WB protocol for Beta-2-Microglobulin antibody 30226-1-APDownload protocol
IF protocol for Beta-2-Microglobulin antibody 30226-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols