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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Beta-2-Microglobulin Monoclonal antibody (66207-1-Ig)

    WB analysis using 66207-1-Ig (same clone as 66207-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66207-1-Ig (Beta-2-Microglobulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66207-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using Beta-2-Microglobulin Monoclonal antibody (66207-1-Ig)

    WB analysis of Raji using 66207-1-Ig (same clone as 66207-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66207-1-Ig (B2M Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66207-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Beta-2-Microglobulin Monoclonal antibody (66207-1-Ig)

    IHC staining of human prostate cancer using 66207-1-Ig (same clone as 66207-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66207-1-Ig (Beta-2-Microglobulin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66207-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Beta-2-Microglobulin Monoclonal antibody (66207-1-Ig)

    IHC staining of human prostate cancer using 66207-1-Ig (same clone as 66207-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66207-1-Ig (Beta-2-Microglobulin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66207-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using Beta-2-Microglobulin Monoclonal antibody (66207-1-Ig)

    IHC staining of human renal cell carcinoma using 66207-1-Ig (same clone as 66207-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66207-1-Ig (B2M Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66207-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using Beta-2-Microglobulin Monoclonal antibody (66207-1-Ig)

    IHC staining of human renal cell carcinoma using 66207-1-Ig (same clone as 66207-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66207-1-Ig (B2M Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66207-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human prostate cancer tissue using Beta-2-Microglobulin Monoclonal antibody (66207-1-Ig)

    IF Staining of human prostate cancer using 66207-1-Ig (same clone as 66207-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using Beta-2-Microglobulin antibody (66207-1-Ig, Clone: 1C3B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66207-1-PBS in a different storage buffer formulation.

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Beta-2-Microglobulin Monoclonal antibody, PBS Only

Beta-2-Microglobulin Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66207-1-PBS
Clone No.1C3B7

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Synonyms

B2M, 1C3B7, HDCMA22P, CDABP0092, Beta-2-microglobulin form pI 5.3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66207-1-PBS targets Beta-2-Microglobulin in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name beta-2-microglobulin
Calculated molecular weight 14 kDa
Observed molecular weight 13 kDa
GenBank accession numberBC032589
Gene Symbol B2M
Gene ID (NCBI) 567
ENSEMBL Gene IDENSG00000166710
RRIDAB_2881598
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP61769
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.