Beta-2-Microglobulin Recombinant antibody
Beta-2-Microglobulin Recombinant Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IP, ELISA
Conjugate
Unconjugated
CloneNo.
240660A12
Cat no : 83683-6-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, HepG2 cells, Raji cells, U-937 cells, Jurkat cells |
Positive IP detected in | A431 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of 83683-6-RR is Beta-2-Microglobulin Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Beta-2-Microglobulin fusion protein Ag4433 相同性解析による交差性が予測される生物種 |
Full Name | beta-2-microglobulin |
Calculated molecular weight | 119 aa, 14 kDa |
Observed molecular weight | 12 kDa |
GenBank accession number | BC032589 |
Gene symbol | B2M |
Gene ID (NCBI) | 567 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions due to shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Beta-2-Microglobulin antibody 83683-6-RR | Download protocol |
IP protocol for Beta-2-Microglobulin antibody 83683-6-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |