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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Beta-2-Microglobulin Recombinant antibody

Beta-2-Microglobulin Recombinant Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

240660A12

Cat no : 83683-6-RR

Synonyms

B2M, 240660A12, HDCMA22P, CDABP0092, Beta-2-microglobulin form pI 5.3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Beta-2-Microglobulin Recombinant antibody (83683-6-RR)

    WB analysis using 83683-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83683-6-RR (Beta-2-Microglobulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A431 cells using Beta-2-Microglobulin Recombinant antibody (83683-6-RR)

    IP experiment of A431 using 83683-6-RR

    IP result of anti-Beta-2-Microglobulin (IP:83683-6-RR, 4ug; Detection:83683-6-RR 1:3000) with A431 cells lysate 2180 ug.

  • Affinity and Kinetic Characterization of 83683-6-RR

    Affinity and Kinetic Characterization of 83683-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83683-6-RR against Human Beta-2-Microglobulin were performed. The affinity constant is 1.18 nM.

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Tested Applications

Positive WB detected inA431 cells, HepG2 cells, Raji cells, U-937 cells, Jurkat cells
Positive IP detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83683-6-RR is Beta-2-Microglobulin Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Beta-2-Microglobulin fusion protein Ag4433 相同性解析による交差性が予測される生物種
Full Name beta-2-microglobulin
Calculated molecular weight 119 aa, 14 kDa
Observed molecular weight 12 kDa
GenBank accession numberBC032589
Gene symbol B2M
Gene ID (NCBI) 567
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions due to shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.

Protocols

Product Specific Protocols
WB protocol for Beta-2-Microglobulin antibody 83683-6-RRDownload protocol
IP protocol for Beta-2-Microglobulin antibody 83683-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols