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Beta Actin Monoclonal antibody, PBS Only

Beta Actin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 60008-1-PBS
Clone No.7D2C10

Host / Isotype

Mouse / IgM

Reactivity

human, mouse, rat, zebrafish, plant

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Actin, ACTB, bate actin, β actin, Beta-actin

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60008-1-PBS targets Beta Actin in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, zebrafish, plant samples.

Tested Reactivity human, mouse, rat, zebrafish, plant
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen Beta Actin fusion protein Ag0297 相同性解析による交差性が予測される生物種
Full Name actin, beta
Calculated molecular weight 375 aa, 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC002409
Gene Symbol Beta Actin
Gene ID (NCBI) 60
RRIDAB_2289225
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP60709
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.

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