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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Beta Arrestin 1 Polyclonal antibody (15361-1-AP)

    WB analysis using 15361-1-AP (same clone as 15361-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15361-1-AP (Beta Arrestin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15361-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using Beta Arrestin 1 Polyclonal antibody (15361-1-AP)

    WB analysis of A549 using 15361-1-AP (same clone as 15361-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 15361-1-AP (ARRB1 antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15361-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-937 cells using Beta Arrestin 1 Polyclonal antibody (15361-1-AP)

    WB analysis of U-937 using 15361-1-AP (same clone as 15361-1-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 15361-1-AP (ARRB1 antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15361-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse lung tissue using Beta Arrestin 1 Polyclonal antibody (15361-1-AP)

    IP experiment of mouse lung using 15361-1-AP (same clone as 15361-1-PBS)

    IP result of anti-Beta Arrestin 1 (IP:15361-1-AP, 4ug; Detection:15361-1-AP 1:500) with mouse lung tissue lysate 4000ug. This data was developed using the same antibody clone with 15361-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Beta Arrestin 1 Polyclonal antibody (15361-1-AP)

    IHC staining of human tonsillitis using 15361-1-AP (same clone as 15361-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15361-1-AP (beta Arrestin 1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15361-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Beta Arrestin 1 Polyclonal antibody (15361-1-AP)

    IHC staining of human tonsillitis using 15361-1-AP (same clone as 15361-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15361-1-AP (beta Arrestin 1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15361-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using Beta Arrestin 1 Polyclonal antibody (15361-1-AP)

    IHC staining of mouse spleen using 15361-1-AP (same clone as 15361-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 15361-1-AP (Beta Arrestin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15361-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using Beta Arrestin 1 Polyclonal antibody (15361-1-AP)

    IHC staining of mouse spleen using 15361-1-AP (same clone as 15361-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 15361-1-AP (Beta Arrestin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15361-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Beta Arrestin 1 Polyclonal antibody (15361-1-AP)

    IHC staining of mouse cerebellum using 15361-1-AP (same clone as 15361-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15361-1-AP (Beta Arrestin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15361-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Beta Arrestin 1 Polyclonal antibody (15361-1-AP)

    IHC staining of mouse cerebellum using 15361-1-AP (same clone as 15361-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15361-1-AP (Beta Arrestin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15361-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Beta Arrestin 1 Polyclonal antibody (15361-1-AP)

    IHC staining of mouse brain using 15361-1-AP (same clone as 15361-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15361-1-AP (Beta Arrestin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15361-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Beta Arrestin 1 Polyclonal antibody (15361-1-AP)

    IHC staining of mouse brain using 15361-1-AP (same clone as 15361-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15361-1-AP (Beta Arrestin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15361-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Beta Arrestin 1 Polyclonal antibody, PBS Only

Beta Arrestin 1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 15361-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15361-1-PBS targets Beta Arrestin 1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Beta Arrestin 1 fusion protein Ag7608 相同性解析による交差性が予測される生物種
Full Name arrestin, beta 1
Calculated molecular weight 47 kDa
Observed molecular weight47-55 kDa
GenBank accession numberBC003636
Gene Symbol Beta Arrestin 1
Gene ID (NCBI) 408
RRIDAB_2060227
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49407
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

β-Arrestins (ARRBs), the best known regulators of G protein-coupled receptor signaling, are versatile and multifunctional adapter proteins that regulate diverse cellular functions, including cell growth, apoptosis and immune responses. Overexpression of beta Arrestin 1 has been found in various cancers, indicating it as a potential therapeutic target for cancer treatment. Recently expression of ARRB1 in saliva has been identified as a candidate circadian biomarker. ARRB1 migrated as a doublet of two bands of 45 and 55 kDa (PMID:28947386).