Beta Arrestin 2 Monoclonal antibody
Beta Arrestin 2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1E8F2
Cat no : 68502-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | LNCaP cells, Jurkat cells, K-562 cells, NIH/3T3 cells, HeLa cells, MCF-7 cells |
Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:20000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
68502-1-Ig targets Beta Arrestin 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Beta Arrestin 2 fusion protein Ag29807 相同性解析による交差性が予測される生物種 |
Full Name | arrestin, beta 2 |
Calculated molecular weight | 46 kDa |
Observed molecular weight | 50 kDa |
GenBank accession number | BC007427 |
Gene symbol | Beta Arrestin 2 |
Gene ID (NCBI) | 409 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Beta Arrestin 2 is a member of arrestin/beta-arrestin protein family, which are expressed at high levels in the central nervous system (CNS) and play a critical role in the regulation of G-protein coupled receptors (GPCRs) including MOR. Beta Arrestin 2 is an adaptor protein that is important for the regulation of receptors belonging to both dopaminergic and opioid systems. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors.
Protocols
Product Specific Protocols | |
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WB protocol for Beta Arrestin 2 antibody 68502-1-Ig | Download protocol |
IHC protocol for Beta Arrestin 2 antibody 68502-1-Ig | Download protocol |
IF protocol for Beta Arrestin 2 antibody 68502-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |