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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Beta Arrestin 2 Recombinant antibody (82780-6-RR)

    WB analysis using 82780-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82780-6-RR (Beta Arrestin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82780-6-RR

    Affinity and Kinetic Characterization of 82780-6-RR

    Biolayer interferometry (BLl) kinetic assays of 82780-6-RR against Human Beta Arrestin 2 were performed. The affinity constant is below 1 pM.

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Beta Arrestin 2 Recombinant antibody

Beta Arrestin 2 Recombinant Antibody for WB, ELISA
Cat No. 82780-6-RR
Clone No.241470F8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ARRB2, β-Arrestin 2, BARR2, Arrestin beta-2, ARR2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inRAW 264.7 cells, mouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82780-6-RR targets Beta Arrestin 2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Beta Arrestin 2 fusion protein Ag0227 相同性解析による交差性が予測される生物種
Full Name arrestin, beta 2
Calculated molecular weight 46 kDa
Observed molecular weight47-50 kDa
GenBank accession numberBC007427
Gene Symbol Beta Arrestin 2
Gene ID (NCBI) 409
RRIDAB_3670548
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP32121
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Beta Arrestin 2 is a member of arrestin/beta-arrestin protein family, which are expressed at high levels in the central nervous system (CNS) and play a critical role in the regulation of G-protein coupled receptors (GPCRs) including MOR. Beta Arrestin 2 is an adaptor protein that is important for the regulation of receptors belonging to both dopaminergic and opioid systems. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors.

Protocols

Product Specific Protocols
WB protocol for Beta Arrestin 2 antibody 82780-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols