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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Beta Catenin Recombinant antibody, PBS Only

Beta Catenin Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Pig, Mouse, Rat, Zebrafish

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2J24

Cat no : 80488-1-PBS

Synonyms

b cat, B-catenin, Beta catenin, Catenin beta 1, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, PRO2286

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Beta Catenin Recombinant antibody (80488-1-RR)

    WB analysis using 80488-1-RR (same clone as 80488-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80488-1-RR (Beta Catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of zebrafish tissue using Beta Catenin Recombinant antibody (80488-1-RR)

    WB analysis of zebrafish using 80488-1-RR (same clone as 80488-1-PBS)

    zebrafish tissue was subjected to SDS PAGE followed by western blot with 80488-1-RR (Beta Catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Beta Catenin Recombinant antibody (80488-1-RR)

    IHC staining of human liver cancer using 80488-1-RR (same clone as 80488-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Beta Catenin Recombinant antibody (80488-1-RR)

    IHC staining of human liver cancer using 80488-1-RR (same clone as 80488-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Beta Catenin Recombinant antibody (80488-1-RR)

    IHC staining of human breast cancer using 80488-1-RR (same clone as 80488-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Beta Catenin Recombinant antibody (80488-1-RR)

    IHC staining of human breast cancer using 80488-1-RR (same clone as 80488-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Beta Catenin Recombinant antibody (80488-1-RR)

    IHC staining of human colon cancer using 80488-1-RR (same clone as 80488-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Beta Catenin Recombinant antibody (80488-1-RR)

    IHC staining of human colon cancer using 80488-1-RR (same clone as 80488-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Beta Catenin Recombinant antibody (80488-1-RR)

    IF Staining of MCF-7 using 80488-1-RR (same clone as 80488-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Beta Catenin antibody (80488-1-RR, Clone: 2J24 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80488-1-PBS targets Beta Catenin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Pig, Mouse, Rat, Zebrafish samples.

Tested Reactivity Human, Pig, Mouse, Rat, Zebrafish
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name catenin (cadherin-associated protein), beta 1, 88kDa
Calculated molecular weight 781 aa, 86 kDa
Observed molecular weight92 kDa
GenBank accession numberBC058926
Gene symbol Beta Catenin
Gene ID (NCBI) 1499
RRIDAB_2918895
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)