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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Beta Galactosidase Monoclonal antibody (66586-1-Ig)

    WB analysis using 66586-1-Ig (same clone as 66586-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66586-1-Ig (Beta Galactosidase antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66586-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using Beta Galactosidase Monoclonal antibody (66586-1-Ig)

    WB analysis of A549 using 66586-1-Ig (same clone as 66586-1-PBS)

    WB result of Beta Galactosidase antibody (66586-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Beta Galactosidase transfected A549 cells. This data was developed using the same antibody clone with 66586-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Beta Galactosidase Monoclonal antibody (66586-1-Ig)

    IHC staining of human kidney using 66586-1-Ig (same clone as 66586-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66586-1-Ig (Beta Galactosidase antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66586-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Beta Galactosidase Monoclonal antibody (66586-1-Ig)

    IHC staining of human kidney using 66586-1-Ig (same clone as 66586-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66586-1-Ig (Beta Galactosidase antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66586-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Beta Galactosidase Monoclonal antibody (66586-1-Ig)

    IF Staining of HepG2 using 66586-1-Ig (same clone as 66586-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Beta Galactosidase antibody (66586-1-Ig, Clone: 4F4F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66586-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Beta Galactosidase Monoclonal antibody, PBS Only

Beta Galactosidase Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66586-1-PBS
Clone No.4F4F4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

GLB1, 4F4F4, Acid beta-galactosidase, Beta-galactosidase, EBP

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66586-1-PBS targets Beta Galactosidase in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Beta Galactosidase fusion protein Ag8069 相同性解析による交差性が予測される生物種
Full Name galactosidase, beta 1
Calculated molecular weight 76 kDa
Observed molecular weight 64-66 kDa, 76-85 kDa
GenBank accession numberBC007493
Gene Symbol GLB1/Beta-galactosidase
Gene ID (NCBI) 2720
RRIDAB_2881946
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP16278
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

GLB1(Beta-galactosidase) is also named as ELNR1 or Lactase. It cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. This protein is identical to the elastin-binding protein (EBP), a major component of the nonintegrin cell surface receptor complex expressed in fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. Defects in GLB1 are the cause of GM1-gangliosidosis type 1 (GM1G1), GM1-gangliosidosis type 2 (GM1G2), GM1-gangliosidosis type 3 (GM1G3) and mucopolysaccharidosis type 4B (MPS4B). GBL1 is synthesized as an 85-kDa precursor that is C-terminally processed into a 64-66 kDa mature form and the released ~20-kDa proteolytic fragment was thought to be degraded (PMID: 10744681). GLB1 has 3 isoforms with MW of 76 kDa, 73 kda and 61 kDa.