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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Beta TrCP Recombinant antibody (84297-1-RR)

    IF Staining of MCF-7 using 84297-1-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Beta TrCP antibody (84297-1-RR, Clone: 241287B8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84297-1-RR

    Affinity and Kinetic Characterization of 84297-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84297-1-RR against Human Beta TrCP were performed. The affinity constant is 2.81 nM.

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Beta TrCP Recombinant antibody

Beta TrCP Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA
Cat No. 84297-1-RR
Clone No.241287B8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

BTRC, 241287B8, FBW1A, F-box/WD repeat-containing protein 1A, F-box and WD repeats protein beta-TrCP

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84297-1-RR targets Beta TrCP in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Beta TrCP fusion protein Ag28739 相同性解析による交差性が予測される生物種
Full Name beta-transducin repeat containing
Calculated molecular weight 605 aa, 69 kDa
GenBank accession numberBC027994
Gene Symbol Beta TRCP
Gene ID (NCBI) 8945
RRIDAB_3671838
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9Y297
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for Beta TrCP antibody 84297-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols