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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Beta galactosidase Recombinant antibody, PBS Only (Detector)

Beta galactosidase Recombinant Antibody for WB, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240788F1

Cat no : 83706-6-PBS

Synonyms

GLB1, ELNR1, EC:3.2.1.23, EBP, Beta-galactosidase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Beta galactosidase Recombinant antibody (83706-6-RR)

    WB analysis using 83706-6-RR (same clone as 83706-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83706-6-RR (Beta galactosidase antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83706-6-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00647-4

    Sandwich ELISA standard curve of MP00647-4

    Sandwich ELISA standard curve of MP00647-4, Human Beta galactosidase Recombinant Matched Antibody Pair - PBS only. 83706-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag7792. 83706-6-PBS was HRP conjugated as the detection antibody. Range: 0.39-12.5 ng/mL.

  • Sample test of MP00647-4

    Sample test of MP00647-4

    The mean Beta galactosidase concentration was determined to be 10.08 ng/mL in HeLa cell extract based on a 2.00 mg/mL extract load.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Beta galactosidase Recombinant antibody (83706-6-RR)

    IF Staining of HepG2 using 83706-6-RR (same clone as 83706-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Beta galactosidase antibody (83706-6-RR, Clone: 240788F1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83706-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using Beta galactosidase Recombinant antibody (83706-6-RR)

    FC experiment of A549 using 83706-6-RR (same clone as 83706-6-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Beta galactosidase Recombinant antibody (83706-6-RR, Clone:240788F1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83706-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83706-6-RR

    Affinity and Kinetic Characterization of 83706-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83706-6-RR against Human Beta galactosidase were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83706-6-PBS targets Beta galactosidase as part of a matched antibody pair:

MP00647-4: 83706-4-PBS capture and 83706-6-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Beta galactosidase fusion protein Ag7792 相同性解析による交差性が予測される生物種
Full Name galactosidase, beta 1
Calculated molecular weight 76 kDa
Observed molecular weight 62-67 kDa
GenBank accession numberBC007493
Gene symbol GLB1/Beta-galactosidase
Gene ID (NCBI) 2720
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GLB1(Beta-galactosidase) is also named as ELNR1 or Lactase. It cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. This protein is identical to the elastin-binding protein (EBP), a major component of the nonintegrin cell surface receptor complex expressed in fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. Defects in GLB1 are the cause of GM1-gangliosidosis type 1 (GM1G1), GM1-gangliosidosis type 2 (GM1G2), GM1-gangliosidosis type 3 (GM1G3) and mucopolysaccharidosis type 4B (MPS4B). GBL1 is synthesized as an 85-kDa precursor that is C-terminally processed into a 64-66 kDa mature form and the released ~20-kDa proteolytic fragment was thought to be degraded (PMID: 10744681). The MW of GLB1 after glycosylation is 100-120 kd. GLB1 is prone to produce homodimers (220-240 kd) and higher multimers (PMID: 3926488). GLB1 has 3 isoforms with MW of 76 kDa, 73 kda, and 61 kDa.