• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Biglycan Monoclonal antibody

Biglycan Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1E3F2

Cat no : 67275-1-Ig

Synonyms

BGN, 1E3F2, PG-S1, PGI, PG S1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of pig skin tissue using Biglycan Monoclonal antibody (67275-1-Ig)

    WB analysis of pig skin using 67275-1-Ig

    pig skin tissue were subjected to SDS PAGE followed by western blot with 67275-1-Ig (Biglycan antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using Biglycan Monoclonal antibody (67275-1-Ig)

    WB analysis of mouse testis using 67275-1-Ig

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 67275-1-Ig (Biglycan antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using Biglycan Monoclonal antibody (67275-1-Ig)

    WB analysis of HepG2 using 67275-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67275-1-Ig (Biglycan antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig colon tissue using Biglycan Monoclonal antibody (67275-1-Ig)

    WB analysis of pig colon using 67275-1-Ig

    pig colon tissue were subjected to SDS PAGE followed by western blot with 67275-1-Ig (Biglycan antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Biglycan Monoclonal antibody (67275-1-Ig)

    IHC staining of human placenta using 67275-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using Biglycan Monoclonal antibody (67275-1-Ig)

    IHC staining of human placenta using 67275-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using Biglycan Monoclonal antibody (67275-1-Ig)

    IHC staining of human lung using 67275-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using Biglycan Monoclonal antibody (67275-1-Ig)

    IHC staining of human lung using 67275-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Biglycan Monoclonal antibody (67275-1-Ig)

    IHC staining of human lung cancer using 67275-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Biglycan Monoclonal antibody (67275-1-Ig)

    IHC staining of human lung cancer using 67275-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Biglycan Monoclonal antibody (67275-1-Ig)

    IHC staining of human colon cancer using 67275-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Biglycan Monoclonal antibody (67275-1-Ig)

    IHC staining of human colon cancer using 67275-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Biglycan Monoclonal antibody (67275-1-Ig)

    IHC staining of human kidney using 67275-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Biglycan Monoclonal antibody (67275-1-Ig)

    IHC staining of human kidney using 67275-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Biglycan Monoclonal antibody (67275-1-Ig)

    IHC staining of human stomach cancer using 67275-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Biglycan Monoclonal antibody (67275-1-Ig)

    IHC staining of human stomach cancer using 67275-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using Biglycan Monoclonal antibody (67275-1-Ig)

    IHC staining of human liver using 67275-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using Biglycan Monoclonal antibody (67275-1-Ig)

    IHC staining of human liver using 67275-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HepG2 cells using Biglycan Monoclonal antibody (67275-1-Ig)

    FC experiment of HepG2 using 67275-1-Ig

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug Biglycan Monoclonal antibody (67275-1-Ig, Clone:1E3F2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive WB detected inpig skin tissue, HepG2 cells, pig colon tissue, mouse testis tissue
Positive IHC detected inhuman kidney tissue, human placenta tissue, human lung tissue, human lung cancer tissue, human colon cancer tissue, human stomach cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67275-1-Ig targets Biglycan in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Biglycan fusion protein Ag8012 相同性解析による交差性が予測される生物種
Full Name biglycan
Calculated molecular weight 42 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC004244
Gene symbol Biglycan
Gene ID (NCBI) 633
RRIDAB_2882544
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Biglycan is a member of the small leucine-rich proteoglycan (SLRP) family (PMID: 18463092). It consists of a core protein of 42 kDa containing leucine-rich repeats (LRRs), to which two chondroitin/dermatan sulfate side chains are covalently bound (PMID: 2212616; 10383378). The tissue-specific chondroitin- or dermatan-sulfate glycosaminoglycan chains of biglycan are attached to amino acid residues at the N-terminus of the core protein (PMID: 22821552). Biglycan is a ubiquitous component of connective tissue matrix and may act as a signaling molecule (PMID: 22821552). Upregulation of biglycan has been reported in multiple types of solid cancer (PMID: 32194659).

Protocols

Product Specific Protocols
WB protocol for Biglycan antibody 67275-1-IgDownload protocol
IHC protocol for Biglycan antibody 67275-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

iScience

Biglycan regulated colorectal cancer progress by modulating enteric neuron-derived IL-10 and abundance of Bacteroides thetaiotaomicron

Authors - Yuyu Xu
View Article
humanIHC

Sci Rep

Exploring the mystery of colon cancer from the perspective of molecular subtypes and treatment

Authors - Wenhong Lu
View Article