BrdU Monoclonal antibody

BrdU Monoclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat and More (1)

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B10E12

Cat no : 66241-1-Ig

Synonyms

5-bromo-2-deoxyuridine, BrDu, BrdUrd, Bromodeoxyuridine, BUdR


"BrdU Antibodies" Comparison

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  • Immunohistochemistry (IHC) staining of mouse spleen tissue using BrdU Monoclonal antibody (66241-1-Ig)

    IHC staining of mouse spleen using 66241-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide (with/without BrdU feeding) using 66241-1-Ig (BrDu Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using BrdU Monoclonal antibody (66241-1-Ig)

    IHC staining of mouse spleen using 66241-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue (with/without BrdU feeding) slide using 66241-1-Ig (BrDu Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using BrdU Monoclonal antibody (66241-1-Ig)

    IHC staining of mouse spleen using 66241-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue (with/without BrdU feeding) slide using 66241-1-Ig (BrDu Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of L-929 cells using BrdU Monoclonal antibody (66241-1-Ig)

    IF Staining of L-929 using 66241-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed L-929 cells using BrdU antibody (66241-1-Ig, Clone: 1B10E12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BrdU Monoclonal antibody (66241-1-Ig)

    IF Staining of HeLa using 66241-1-Ig

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 66241-1-Ig (BrdU antibody) at dilution of 1:300 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BrdU Monoclonal antibody (66241-1-Ig)

    IF Staining of HeLa using 66241-1-Ig

    Immunofluorescent analysis of HeLa cells (treated with 0.03 mg/ml BrdU for 2 hours) using 66241-1-Ig(BrdU antibody) at dilution of 1:300 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, L-929 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66241-1-Ig targets BrdU in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Compound 相同性解析による交差性が予測される生物種
Full Name BrdU
Gene symbol
Gene ID (NCBI)
RRIDAB_2881630
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has 0mitogenic, transcriptional and translational effects on cells that incorporate it. This antibody does not cross react with Thymidine.

Protocol for IHC:
https://www.ptgcn.com/protocol/66241-1-IgIHC.pdf

Protocol for IF:
https://www.ptgcn.com/protocol/66241-1-IgIF.pdf

Protocols

Product Specific Protocols
IHC protocol for BrdU antibody 66241-1-IgDownload protocol
IF protocol for BrdU antibody 66241-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Cell Metab

The browning and mobilization of subcutaneous white adipose tissue supports efficient skin repair

Authors - Junrong Cai
mouseIHC,IF

Nat Cell Biol

LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis.

Authors - Pingping Zhu
mouseIF

Sci Transl Med

FoxO transcription factors modulate autophagy and proteoglycan 4 in cartilage homeostasis and osteoarthritis.

Authors - Tokio Matsuzaki
mouseIF

Cell Res

A novel m6A reader Prrc2a controls oligodendroglial specification and myelination.

Authors - Rong Wu
humanIF

Nat Commun

MRNIP condensates promote DNA double-strand break sensing and end resection.

Authors - Yun-Long Wang
mouseIF

Nat Commun

Replication stress triggers microsatellite destabilization and hypermutation leading to clonal expansion in vitro.

Authors - Yusuke Matsuno