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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C10orf58 Polyclonal antibody

C10orf58 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20446-1-AP

Synonyms

C10orf58, F213A, FAM213A, PSEC0139, UNQ611/PRO1198, UPF0765 protein C10orf58

Validation Data Gallery

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  • Western Blot (WB) analysis of ROS1728 cells using C10orf58 Polyclonal antibody (20446-1-AP)

    WB analysis of ROS1728 using 20446-1-AP

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 20446-1-AP (C10orf58 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin tissue using C10orf58 Polyclonal antibody (20446-1-AP)

    IHC staining of human skin using 20446-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 20446-1-AP (C10orf58 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using C10orf58 Polyclonal antibody (20446-1-AP)

    IHC staining of human skin using 20446-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 20446-1-AP (C10orf58 antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of ROS1728 cells using C10orf58 Polyclonal antibody (20446-1-AP)

    IF Staining of ROS1728 using 20446-1-AP

    Immunofluorescent analysis of ROS1728 cells using 20446-1-AP (C10orf58 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inROS1728 cells
Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inROS1728 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20446-1-AP is C10orf58 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C10orf58 fusion protein Ag14272 相同性解析による交差性が予測される生物種
Full Name chromosome 10 open reading frame 58
Calculated molecular weight 229 aa, 26 kDa
Observed molecular weight 24-30 kDa
GenBank accession numberBC005871
Gene symbol C10orf58
Gene ID (NCBI) 84293
RRIDAB_2878687
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C10orf58, also name as FAM213A and PAMM, is involved in redox regulation of the cell. It acts as an antioxidant. C10orf58 inhibits TNFSF11-induced NFKB1 and JUN activation and osteoclast differentiation. It may affect bone resorption and help to maintain bone mass. The MW of C10orf58 is 24-30 kDa.

Protocols

Product Specific Protocols
WB protocol for C10orf58 antibody 20446-1-APDownload protocol
IHC protocol for C10orf58 antibody 20446-1-APDownload protocol
IF protocol for C10orf58 antibody 20446-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols