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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C11orf49 Polyclonal antibody

C11orf49 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20195-1-AP

Synonyms

C11orf49, UPF0705 protein C11orf49

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse lung tissue using C11orf49 Polyclonal antibody (20195-1-AP)

    WB analysis of mouse lung using 20195-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 20195-1-AP (C11orf49 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using C11orf49 Polyclonal antibody (20195-1-AP)

    WB analysis of human brain using 20195-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20195-1-AP (C11orf49 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using C11orf49 Polyclonal antibody (20195-1-AP)

    IHC staining of human colon using 20195-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 20195-1-AP (C11orf49 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using C11orf49 Polyclonal antibody (20195-1-AP)

    IHC staining of human colon using 20195-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 20195-1-AP (C11orf49 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using C11orf49 Polyclonal antibody (20195-1-AP)

    IF Staining of HepG2 using 20195-1-AP

    Immunofluorescent analysis of HepG2 cells, using C11orf49 antibody 20195-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inmouse lung tissue, human brain tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20195-1-AP is C11orf49 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C11orf49 fusion protein Ag14123 相同性解析による交差性が予測される生物種
Full Name chromosome 11 open reading frame 49
Calculated molecular weight 331 aa, 37 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC001860
Gene symbol C11orf49
Gene ID (NCBI) 79096
RRIDAB_10646431
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for C11orf49 antibody 20195-1-APDownload protocol
IHC protocol for C11orf49 antibody 20195-1-APDownload protocol
IF protocol for C11orf49 antibody 20195-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Cell

Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.

Authors - Edward L Huttlin
View Article