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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

APC15 Polyclonal antibody

APC15 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20409-1-AP

Synonyms

C11orf51, HSPC020, Anaphase-promoting complex subunit 15, ANAPC15

Validation Data Gallery

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  • Western Blot (WB) analysis of HT-29 cells using APC15 Polyclonal antibody (20409-1-AP)

    WB analysis of HT-29 using 20409-1-AP

    HT-29 cells were subjected to SDS PAGE followed by western blot with 20409-1-AP (APC15 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using APC15 Polyclonal antibody (20409-1-AP)

    WB analysis of HEK-293 using 20409-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20409-1-AP (APC15 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using APC15 Polyclonal antibody (20409-1-AP)

    IHC staining of human placenta using 20409-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20409-1-AP (APC15 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using APC15 Polyclonal antibody (20409-1-AP)

    IHC staining of mouse spleen using 20409-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 20409-1-AP (APC15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat adrenal gland tissue using APC15 Polyclonal antibody (20409-1-AP)

    IHC staining of rat adrenal gland using 20409-1-AP

    Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 20409-1-AP (APC15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat lung tissue using APC15 Polyclonal antibody (20409-1-AP)

    IHC staining of rat lung using 20409-1-AP

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 20409-1-AP (APC15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using APC15 Polyclonal antibody (20409-1-AP)

    IHC staining of human lung using 20409-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 20409-1-AP (APC15 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using APC15 Polyclonal antibody (20409-1-AP)

    IHC staining of human lung using 20409-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 20409-1-AP (APC15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse adrenal gland tissue using APC15 Polyclonal antibody (20409-1-AP)

    IHC staining of mouse adrenal gland using 20409-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 20409-1-AP (APC15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using APC15 Polyclonal antibody (20409-1-AP)

    IHC staining of mouse lung using 20409-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20409-1-AP (APC15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using APC15 Polyclonal antibody (20409-1-AP)

    IHC staining of human pancreas using 20409-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 20409-1-AP (C11orf51 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using APC15 Polyclonal antibody (20409-1-AP)

    IHC staining of human pancreas using 20409-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 20409-1-AP (C11orf51 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inHT-29 cells, HEK-293 cells
Positive IHC detected inhuman placenta tissue, human lung tissue, human pancreas tissue, mouse adrenal gland tissue, mouse lung tissue, mouse spleen tissue, rat adrenal gland tissue, rat lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20409-1-AP targets APC15 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APC15 fusion protein Ag14237 相同性解析による交差性が予測される生物種
Full Name chromosome 11 open reading frame 51
Calculated molecular weight 121 aa, 14 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC005156
Gene symbol APC15
Gene ID (NCBI) 25906
RRIDAB_10694279
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for APC15 antibody 20409-1-APDownload protocol
IHC protocol for APC15 antibody 20409-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols