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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C11orf54 Polyclonal antibody

C11orf54 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23251-1-AP

Synonyms

C11orf54, Ester hydrolase C11orf54, PTD012

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of rat liver tissue using C11orf54 Polyclonal antibody (23251-1-AP)

    WB analysis of rat liver using 23251-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 23251-1-AP (C11orf54 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using C11orf54 Polyclonal antibody (23251-1-AP)

    IHC staining of mouse liver using 23251-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 23251-1-AP (C11orf54 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using C11orf54 Polyclonal antibody (23251-1-AP)

    IHC staining of mouse liver using 23251-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 23251-1-AP (C11orf54 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

Positive WB detected inrat liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23251-1-AP is C11orf54 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C11orf54 fusion protein Ag19731 相同性解析による交差性が予測される生物種
Full Name chromosome 11 open reading frame 54
Calculated molecular weight 315 aa, 35 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC007110
Gene symbol C11orf54
Gene ID (NCBI) 28970
RRIDAB_2879239
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for C11orf54 antibody 23251-1-APDownload protocol
IHC protocol for C11orf54 antibody 23251-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Commun Biol

C11orf54 promotes DNA repair via blocking CMA-mediated degradation of HIF1A

Authors - Junyang Tan
View Article