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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C11orf57 Polyclonal antibody

C11orf57 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21181-1-AP

Synonyms

C11orf57

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse brain tissue using C11orf57 Polyclonal antibody (21181-1-AP)

    WB analysis of mouse brain using 21181-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21181-1-AP (C11orf57 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using C11orf57 Polyclonal antibody (21181-1-AP)

    IHC staining of human breast cancer using 21181-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21181-1-AP (C11orf57 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using C11orf57 Polyclonal antibody (21181-1-AP)

    IHC staining of human breast cancer using 21181-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21181-1-AP (C11orf57 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 21181-1-AP is C11orf57 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C11orf57 fusion protein Ag15471 相同性解析による交差性が予測される生物種
Full Name chromosome 11 open reading frame 57
Calculated molecular weight 293 aa, 34 kDa
Observed molecular weight 30-34 kDa
GenBank accession numberBC030546
Gene symbol C11orf57
Gene ID (NCBI) 55216
RRIDAB_2878825
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C11orf57 has three isoforms with MW 30-34 kDa.

Protocols

Product Specific Protocols
WB protocol for C11orf57 antibody 21181-1-APDownload protocol
IHC protocol for C11orf57 antibody 21181-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

FACS-based genome-wide CRISPR screens define key regulators of DNA damage signaling pathways

Authors - Min Huang
View Article