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  • Featured Product
  • KD/KO Validated

Hikeshi Polyclonal antibody

Hikeshi Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 20524-1-AP

Synonyms

C11orf73, Protein Hikeshi, L7RN6, HSPC248, HSPC179

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using Hikeshi Polyclonal antibody (20524-1-AP)

    WB analysis of PC-3 using 20524-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 20524-1-AP (Hikeshi antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using Hikeshi Polyclonal antibody (20524-1-AP)

    WB analysis of A375 using 20524-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 20524-1-AP (Hikeshi antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using Hikeshi Polyclonal antibody (20524-1-AP)

    WB analysis of mouse brain using 20524-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20524-1-AP (Hikeshi antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using Hikeshi Polyclonal antibody (20524-1-AP)

    IP experiment of mouse brain using 20524-1-AP

    IP result of anti-Hikeshi (IP:20524-1-AP, 4ug; Detection:20524-1-AP 1:500) with mouse brain tissue lysate 3000ug.

  • Immunohistochemistry (IHC) staining of human lung tissue using Hikeshi Polyclonal antibody (20524-1-AP)

    IHC staining of human lung using 20524-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 20524-1-AP (Hikeshi antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using Hikeshi Polyclonal antibody (20524-1-AP)

    IHC staining of human lung using 20524-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 20524-1-AP (Hikeshi antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using Hikeshi Polyclonal antibody (20524-1-AP)

    IF Staining of A375 using 20524-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A375 cells using Hikeshi antibody (20524-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inPC-3 cells, A375 cells, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20524-1-AP targets Hikeshi in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Hikeshi fusion protein Ag14376 相同性解析による交差性が予測される生物種
Full Name chromosome 11 open reading frame 73
Calculated molecular weight 197 aa, 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC006476
Gene symbol Hikeshi
Gene ID (NCBI) 51501
RRIDAB_10666160
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Hikeshi antibody 20524-1-APDownload protocol
IHC protocol for Hikeshi antibody 20524-1-APDownload protocol
IF protocol for Hikeshi antibody 20524-1-APDownload protocol
IP protocol for Hikeshi antibody 20524-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,ratWB,IHC,IF

Dev Cell

Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination

Authors - Li Li
  • KO Validated
View Article
humanWB

Life Sci Alliance

Lack of Hikeshi activates HSF1 activity under normal conditions and disturbs the heat-shock response.

Authors - Shingo Kose
View Article
mouseIF

Medicines (Basel)

The Infantile Leukoencephalopathy-Associated Mutation of C11ORF73/HIKESHI Proteins Generates de novo Interactive Activity with Filamin A, Inhibiting Oligodendroglial Cell Morphological Differentiation.

Authors - Kohei Hattori
View Article
humanWB,IF

Am J Med Genet A

A novel missense variant in HIKESHI: Clinical phenotype, in vitro functional testing, and potential for gene therapy

Authors - Eric J Mallack
View Article