Validation Data Gallery
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Tested Applications
| Positive WB detected in | PC-3 cells, A375 cells, mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A375 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
| IF | See 3 publications below |
Product Information
20524-1-AP targets Hikeshi in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Hikeshi fusion protein Ag14376 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 11 open reading frame 73 |
| Calculated molecular weight | 197 aa, 22 kDa |
| Observed molecular weight | 22 kDa |
| GenBank accession number | BC006476 |
| Gene Symbol | Hikeshi |
| Gene ID (NCBI) | 51501 |
| RRID | AB_10666160 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q53FT3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Hikeshi antibody 20524-1-AP | Download protocol |
| IHC protocol for Hikeshi antibody 20524-1-AP | Download protocol |
| IF protocol for Hikeshi antibody 20524-1-AP | Download protocol |
| IP protocol for Hikeshi antibody 20524-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Dev Cell Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination
| ||
Life Sci Alliance Lack of Hikeshi activates HSF1 activity under normal conditions and disturbs the heat-shock response. | ||
Medicines (Basel) The Infantile Leukoencephalopathy-Associated Mutation of C11ORF73/HIKESHI Proteins Generates de novo Interactive Activity with Filamin A, Inhibiting Oligodendroglial Cell Morphological Differentiation. | ||
Am J Med Genet A A novel missense variant in HIKESHI: Clinical phenotype, in vitro functional testing, and potential for gene therapy |