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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C12orf29 Polyclonal antibody

C12orf29 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 31647-1-AP

Synonyms

Rnl, RNA Ligase 1, RNA Ligase

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using C12orf29 Polyclonal antibody (31647-1-AP)

    WB analysis using 31647-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31647-1-AP (C12orf29 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using C12orf29 Polyclonal antibody (31647-1-AP)

    IHC staining of mouse small intestine using 31647-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 31647-1-AP (C12orf29 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using C12orf29 Polyclonal antibody (31647-1-AP)

    IHC staining of mouse small intestine using 31647-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 31647-1-AP (C12orf29 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using C12orf29 Polyclonal antibody (31647-1-AP)

    IF Staining of HeLa using 31647-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using C12orf29 antibody (31647-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inHL-60 cells, HeLa cells, U-87 MG cells
Positive IHC detected inmouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31647-1-AP targets C12orf29 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C12orf29 fusion protein Ag35681 相同性解析による交差性が予測される生物種
Full Name chromosome 12 open reading frame 29
Calculated molecular weight37kDa，325aa
Observed molecular weight37 kDa
GenBank accession numberNM_001009894.2
Gene symbol C12orf29
Gene ID (NCBI) 91298
RRIDAB_3670063
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C12orf29, also known as RLIG1, is a 37 kDa human protein consisting of 325 amino acids. It catalyzes ATP-dependent RNA ligation via a three-step mechanism involving tandem auto- and RNA AMPylation (PMID: 36792600).

Protocols

Product Specific Protocols
WB protocol for C12orf29 antibody 31647-1-APDownload protocol
IHC protocol for C12orf29 antibody 31647-1-APDownload protocol
IF protocol for C12orf29 antibody 31647-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols