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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C13orf1 Polyclonal antibody

C13orf1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26510-1-AP

Synonyms

SPRYD7, SPRY7, SPRY domain-containing protein 7, CLLD6

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using C13orf1 Polyclonal antibody (26510-1-AP)

    WB analysis of mouse brain using 26510-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 26510-1-AP (C13orf1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using C13orf1 Polyclonal antibody (26510-1-AP)

    IHC staining of human lymphoma using 26510-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 26510-1-AP (C13orf1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using C13orf1 Polyclonal antibody (26510-1-AP)

    IHC staining of human lymphoma using 26510-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 26510-1-AP (C13orf1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using C13orf1 Polyclonal antibody (26510-1-AP)

    IF Staining of PC-12 using 26510-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using C13orf1 antibody (26510-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26510-1-AP targets C13orf1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C13orf1 fusion protein Ag23487 相同性解析による交差性が予測される生物種
Full Name chromosome 13 open reading frame 1
Observed molecular weight 17 kDa, 20 kDa
GenBank accession numberBC022519
Gene symbol C13orf1
Gene ID (NCBI) 57213
RRIDAB_2880536
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C13orf1, also named as SPRYD7 and CLLD6, is involved in hematopoiesis via NOTCH signaling. C13orf1 plays an important role in the pathogenesis of B-CLL. There're some isoforms of C13orf1 with MW 15-17 kDa and 19-22 kDa.3

Protocols

Product Specific Protocols
WB protocol for C13orf1 antibody 26510-1-APDownload protocol
IHC protocol for C13orf1 antibody 26510-1-APDownload protocol
IF protocol for C13orf1 antibody 26510-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols