C13orf18 Polyclonal antibody
C13orf18 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 21183-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human skeletal muscle tissue, MCF-7 cells |
Positive IHC detected in | human testis tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 2 publications below |
Product Information
The immunogen of 21183-1-AP is C13orf18 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | C13orf18 fusion protein Ag15474 相同性解析による交差性が予測される生物種 |
Full Name | chromosome 13 open reading frame 18 |
Calculated molecular weight | 662 aa, 73 kDa |
Observed molecular weight | 66 kDa |
GenBank accession number | BC032311 |
Gene symbol | C13orf18 |
Gene ID (NCBI) | 80183 |
RRID | AB_10733098 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for C13orf18 antibody 21183-1-AP | Download protocol |
IHC protocol for C13orf18 antibody 21183-1-AP | Download protocol |
IF protocol for C13orf18 antibody 21183-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nature Architecture of the human interactome defines protein communities and disease networks. | ||
Precis Clin Med Blockade of Syk modulates neutrophil immune-responses via the mTOR/RUBCNL-dependent autophagy pathway to alleviate intestinal inflammation in ulcerative colitis |