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Validation Data Gallery

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  • Western Blot (WB) analysis of SGC-7901 cells using Vertnin Polyclonal antibody (24824-1-AP)

    WB analysis of SGC-7901 using 24824-1-AP

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 24824-1-AP (Vertnin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Vertnin Polyclonal antibody (24824-1-AP)

    IHC staining of human stomach cancer using 24824-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24824-1-AP (Vertnin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using Vertnin Polyclonal antibody (24824-1-AP)

    IHC staining of human urothelial carcinoma using 24824-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 24824-1-AP (Vertnin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Vertnin Polyclonal antibody

Vertnin Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24824-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

C14orf115, VRTN

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSGC-7901 cells
Positive IHC detected inhuman urothelial carcinoma tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24824-1-AP targets Vertnin in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Vertnin fusion protein Ag20678 相同性解析による交差性が予測される生物種
Full Name chromosome 14 open reading frame 115
Calculated molecular weight 702 aa, 78 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC053325
Gene Symbol Vertnin
Gene ID (NCBI) 55237
RRIDAB_2879745
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H8Y1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vertnin, also named as VRTN and C14orf115, belongs to the vertnin family. It is an essential factor for development of the embryo in a wide range of organisms.

Protocols

Product Specific Protocols
WB protocol for Vertnin antibody 24824-1-APDownload protocol
IHC protocol for Vertnin antibody 24824-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols