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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C14orf126 Polyclonal antibody

C14orf126 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23923-1-AP

Synonyms

EC:3.1.1.96, D-tyrosyl-tRNA(Tyr) deacylase 2, DTD2, D-aminoacyl-tRNA deacylase 2, ATD

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C14orf126 Polyclonal antibody (23923-1-AP)

    WB analysis using 23923-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23923-1-AP (C14orf126 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using C14orf126 Polyclonal antibody (23923-1-AP)

    WB analysis using 23923-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23923-1-AP (C14orf126 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using C14orf126 Polyclonal antibody (23923-1-AP)

    IF Staining of A549 using 23923-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using C14orf126 antibody (23923-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inA549 cells, mouse cerebellum tissue, HepG2 cells, mouse lung tissue
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23923-1-AP targets C14orf126 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C14orf126 fusion protein Ag21053 相同性解析による交差性が予測される生物種
Full Name chromosome 14 open reading frame 126
Calculated molecular weight 168 aa, 19 kDa
Observed molecular weight19 kDa
GenBank accession numberBC010618
Gene symbol C14orf126
Gene ID (NCBI) 112487
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C14orf126, also named D-aminoacyl-tRNA deacylase 2 (DTD2), can deacylate L-Ala due to a relaxed specificity for substrate chirality caused by the trans conformation of the Gly-Pro motif in the active site. Plants are unique in possessing two distinct chiral proofreading systems, D-aminoacyl-tRNA deacylase1 (DTD1) and DTD2, of bacterial and archaeal origins, respectively.

Protocols

Product Specific Protocols
WB protocol for C14orf126 antibody 23923-1-APDownload protocol
IF protocol for C14orf126 antibody 23923-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols