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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C14orf138 Polyclonal antibody

C14orf138 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24722-1-AP

Synonyms

C14orf138

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using C14orf138 Polyclonal antibody (24722-1-AP)

    WB analysis of PC-3 using 24722-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 24722-1-AP (C14orf138 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using C14orf138 Polyclonal antibody (24722-1-AP)

    WB analysis of HepG2 using 24722-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 24722-1-AP (C14orf138 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using C14orf138 Polyclonal antibody (24722-1-AP)

    WB analysis of human testis using 24722-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 24722-1-AP (C14orf138 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using C14orf138 Polyclonal antibody (24722-1-AP)

    IHC staining of mouse testis using 24722-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24722-1-AP (C14orf138 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using C14orf138 Polyclonal antibody (24722-1-AP)

    IHC staining of mouse testis using 24722-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24722-1-AP (C14orf138 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inPC-3 cells, HepG2 cells, human testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24722-1-AP targets C14orf138 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C14orf138 fusion protein Ag20154 相同性解析による交差性が予測される生物種
Full Name chromosome 14 open reading frame 138
Calculated molecular weight 229 aa, 26 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC105118
Gene symbol C14orf138
Gene ID (NCBI) 79609
RRIDAB_2879689
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C14orf138, also named as VCPKMT or METTL21D, is 229 amino acid protein, which belongs to the methyltransferase superfamily. C14orf138 localizes in the cytoplasm and interacts with ALKBH6. C14orf138 as a Protein-lysine N-methyltransferase that specifically trimethylates 'Lys-315' of VCP/p97; this modification may decrease VCP ATPase activity. C14orf138 exists as five isoforms. The calculated molecular weight of C14orf138 is 26 kDa,but we always detected a 46-50 kDa portein by western blot.

Protocols

Product Specific Protocols
WB protocol for C14orf138 antibody 24722-1-APDownload protocol
IHC protocol for C14orf138 antibody 24722-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols