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KD/KO Validated

SLIRP Polyclonal antibody

Name: SLIRP Polyclonal antibody
Brand: Proteintech
SKU: 26006-1-AP

SLIRP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 26006-1-AP

Synonyms

SRA stem-loop-interacting RNA-binding protein, mitochondrial, PD04872, DC50, DC23, C14orf156

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 26006-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26006-1-AP (SLIRP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of MDA-MB-453s using 26006-1-AP
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 26006-1-AP (SLIRP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 26006-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 26006-1-AP (SLIRP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 26006-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 26006-1-AP (SLIRP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human kidney using 26006-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26006-1-AP (SLIRP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 26006-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26006-1-AP (SLIRP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary cancer using 26006-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 26006-1-AP (SLIRP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 26006-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26006-1-AP (SLIRP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 26006-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26006-1-AP (SLIRP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 26006-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26006-1-AP (SLIRP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of PC-3 using 26006-1-AP
Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using SLIRP antibody (26006-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of PC-3 using 26006-1-AP
Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using SLIRP antibody (26006-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 26006-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, PC-3 cells, MDA-MB-453s cells
Positive IHC detected in	human ovary cancer tissue, human kidney tissue, human stomach cancer tissue, mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	PC-3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 5 publications below
IF	See 1 publications below
CoIP	See 1 publications below

Product Information

26006-1-AP targets SLIRP in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SLIRP fusion protein Ag22945 相同性解析による交差性が予測される生物種
Full Name	chromosome 14 open reading frame 156
Calculated molecular weight	109 aa, 12 kDa
Observed molecular weight	12 kDa
GenBank accession number	BC017895
Gene symbol	SLIRP
Gene ID (NCBI)	81892
RRID	AB_2880332
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SLIRP, also named as C14orf156, DC23, DC50 and PD04872. It is an RNA-binding protein on steroid receptor RNA activator (SRA), and its expression is ubiquitous in human normal tissues. SLIRP can affect mitochondrial mRNA transcription and energy conversion. In addition, the loss or weakening of SLIRP destroys part of the structure of mitochondria, affects mitochondria energy metabolism, and increases sperm or cell oxidative stress damage (PMID: 33150185). SLIRP has 2 isoforms with the molecular mass of 12 kDa.

Protocols

Product Specific Protocols
WB protocol for SLIRP antibody 26006-1-AP	Download protocol
IHC protocol for SLIRP antibody 26006-1-AP	Download protocol
IF protocol for SLIRP antibody 26006-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF,CoIP	Redox Biol LRPPRC regulates redox homeostasis via the circANKHD1/FOXM1 axis to enhance bladder urothelial carcinoma tumorigenesis. Authors - Wen-Su Wei KD Validated View Article
human	WB	Methods Mol Biol Sucrose Gradient Analysis of Human Mitochondrial Ribosomes and RNA Authors - Kah Ying Ng View Article
human	WB	J Transl Med A traditional gynecological medicine inhibits ovarian cancer progression and eliminates cancer stem cells via the LRPPRC-OXPHOS axis Authors - Ruibin Jiang View Article
human	WB	Int J Mol Sci Mitochondrial Protein SLIRP Affects Biosynthesis of Cytochrome c Oxidase Subunits in HEK293T Cells Authors - Mariia V Baleva View Article
human	WB	Cell Death Differ C-IGF1R encoded by cIGF1R acts as a molecular switch to restrict mitophagy of drug-tolerant persister tumour cells in non-small cell lung cancer Authors - Hui Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLIRP Polyclonal antibody

SLIRP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 26006-1-AP

WB analysis of MDA-MB-453s using 26006-1-AP

WB analysis of HeLa using 26006-1-AP

WB analysis of PC-3 using 26006-1-AP

IHC staining of human kidney using 26006-1-AP

IHC staining of mouse testis using 26006-1-AP

IHC staining of human ovary cancer using 26006-1-AP

IHC staining of human stomach cancer using 26006-1-AP

IHC staining of mouse testis using 26006-1-AP

IHC staining of human kidney using 26006-1-AP

IF Staining of PC-3 using 26006-1-AP

IF Staining of PC-3 using 26006-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

LRPPRC regulates redox homeostasis via the circANKHD1/FOXM1 axis to enhance bladder urothelial carcinoma tumorigenesis.

Sucrose Gradient Analysis of Human Mitochondrial Ribosomes and RNA

A traditional gynecological medicine inhibits ovarian cancer progression and eliminates cancer stem cells via the LRPPRC-OXPHOS axis

Mitochondrial Protein SLIRP Affects Biosynthesis of Cytochrome c Oxidase Subunits in HEK293T Cells

C-IGF1R encoded by cIGF1R acts as a molecular switch to restrict mitophagy of drug-tolerant persister tumour cells in non-small cell lung cancer