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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using C14orf39 Polyclonal antibody (22664-1-AP)

    WB analysis of Jurkat using 22664-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22664-1-AP (C14orf39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse colon tissue using C14orf39 Polyclonal antibody (22664-1-AP)

    WB analysis of mouse colon using 22664-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 22664-1-AP (C14orf39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SKOV-3 cells using C14orf39 Polyclonal antibody (22664-1-AP)

    WB analysis of SKOV-3 using 22664-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 22664-1-AP (C14orf39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using C14orf39 Polyclonal antibody (22664-1-AP)

    WB analysis of HeLa using 22664-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22664-1-AP (C14orf39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using C14orf39 Polyclonal antibody (22664-1-AP)

    IHC staining of human skeletal muscle using 22664-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22664-1-AP (C14orf39 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using C14orf39 Polyclonal antibody (22664-1-AP)

    IHC staining of human skeletal muscle using 22664-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22664-1-AP (C14orf39 Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using C14orf39 Polyclonal antibody (22664-1-AP)

    IF Staining of mouse testis using 22664-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using C14orf39 antibody (22664-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using C14orf39 Polyclonal antibody (22664-1-AP)

    IF Staining of MCF-7 using 22664-1-AP

    Immunofluorescent analysis of MCF-7 cells using 22664-1-AP (C14orf39 antibody) at dilution of 1:50.

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  • Featured Product
  • KD/KO Validated

C14orf39 Polyclonal antibody

C14orf39 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 22664-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SIX6OS1, Six6 opposite strand transcript 1, Protein SIX6OS1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, mouse colon tissue, SKOV-3 cells
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22664-1-AP targets C14orf39 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C14orf39 fusion protein Ag18589 相同性解析による交差性が予測される生物種
Full Name chromosome 14 open reading frame 39
Calculated molecular weight 587 aa, 68 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC125070
Gene Symbol C14orf39
Gene ID (NCBI) 317761
RRIDAB_2879146
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N1H7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for C14orf39 antibody 22664-1-APDownload protocol
IHC protocol for C14orf39 antibody 22664-1-APDownload protocol
IF protocol for C14orf39 antibody 22664-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nat Commun

C14ORF39/SIX6OS1 is a constituent of the synaptonemal complex and is essential for mouse fertility.

Authors - Laura Gómez-H
  • KO Validated
View Article