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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C14orf94 Polyclonal antibody

C14orf94 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20115-1-AP

Synonyms

C14orf94, HAUS4

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using C14orf94 Polyclonal antibody (20115-1-AP)

    WB analysis of PC-3 using 20115-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 20115-1-AP (C14orf94 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skin tissue using C14orf94 Polyclonal antibody (20115-1-AP)

    WB analysis of mouse skin using 20115-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 20115-1-AP (C14orf94 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung tissue using C14orf94 Polyclonal antibody (20115-1-AP)

    IHC staining of human lung using 20115-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 20115-1-AP (C14orf94 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using C14orf94 Polyclonal antibody (20115-1-AP)

    IHC staining of human lung using 20115-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 20115-1-AP (C14orf94 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using C14orf94 Polyclonal antibody (20115-1-AP)

    IHC staining of human skin using 20115-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 20115-1-AP (C14orf94 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using C14orf94 Polyclonal antibody (20115-1-AP)

    IHC staining of human skin using 20115-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 20115-1-AP (C14orf94 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inPC-3 cells, mouse skin tissue
Positive IHC detected inhuman lung tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20115-1-AP is C14orf94 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C14orf94 fusion protein Ag13986 相同性解析による交差性が予測される生物種
Full Name chromosome 14 open reading frame 94
Calculated molecular weight 363 aa, 42 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC001916
Gene symbol C14orf94
Gene ID (NCBI) 54930
RRIDAB_10638430
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HAUS4, also named as C14orf94, has four isoforms with MW 27-29 kDa, 37 kDa and 42 kDa.

Protocols

Product Specific Protocols
WB protocol for C14orf94 antibody 20115-1-APDownload protocol
IHC protocol for C14orf94 antibody 20115-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols