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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C15orf42 Polyclonal antibody (30664-1-AP)

    WB analysis using 30664-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30664-1-AP (C15orf42 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using C15orf42 Polyclonal antibody (30664-1-AP)

    IF Staining of A431 using 30664-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using C15orf42 antibody (30664-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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C15orf42 Polyclonal antibody

C15orf42 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30664-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

C15orf42, FLJ41618, TICRR, Treslin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30664-1-AP targets C15orf42 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C15orf42 fusion protein Ag33632 相同性解析による交差性が予測される生物種
Full Name chromosome 15 open reading frame 42
Observed molecular weight210-250 kDa
GenBank accession numberBC033209
Gene Symbol C15orf42
Gene ID (NCBI) 90381
RRIDAB_3669748
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z2Z1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C15orf42 (TOPBP1 interacting checkpoint and replication regulator), also known as Treslin. It is expected to be located in cytosol and nucleoplasm, the protein is mainly expressed in bone marrow, esophagus, and lymphoid tissue. The protein C15orf42 enables chromatin binding activity and it is involved in regulation of DNA-dependent DNA replication initiation. And the calculated molecular weight of C15orf42 is 210 kDa, the 250 kDa band has been reported in the literature (PMID: 30037903).

Protocols

Product Specific Protocols
WB protocol for C15orf42 antibody 30664-1-APDownload protocol
IF protocol for C15orf42 antibody 30664-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols