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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C15orf58 Polyclonal antibody, PBS Only

C15orf58 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 25447-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using C15orf58 Polyclonal antibody (25447-1-AP)

    WB analysis of PC-3 using 25447-1-AP (same clone as 25447-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25447-1-AP (C15orf58 Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25447-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using C15orf58 Polyclonal antibody (25447-1-AP)

    WB analysis of PC-3 using 25447-1-AP (same clone as 25447-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25447-1-AP (C15orf58 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25447-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using C15orf58 Polyclonal antibody (25447-1-AP)

    WB analysis of mouse brain using 25447-1-AP (same clone as 25447-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25447-1-AP (C15orf58 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25447-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using C15orf58 Polyclonal antibody (25447-1-AP)

    IF Staining of PC-3 using 25447-1-AP (same clone as 25447-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 25447-1-AP (C15orf58 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25447-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25447-1-PBS targets C15orf58 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C15orf58 fusion protein Ag20215 相同性解析による交差性が予測される生物種
Full Name chromosome 15 open reading frame 58
Calculated molecular weight 385 aa, 42 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC127821
Gene symbol C15orf58
Gene ID (NCBI) 390637
RRIDAB_2880085
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.