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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VPS35L Polyclonal antibody

VPS35L Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26923-1-AP

Synonyms

UPF0505 protein C16orf62, RTSC3, Esophageal cancer-associated protein, EC97, C16orf62

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VPS35L Polyclonal antibody (26923-1-AP)

    WB analysis using 26923-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26923-1-AP (VPS35L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inK-562 cells, MOLT-4 cells, Raji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26923-1-AP targets VPS35L in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS35L fusion protein Ag25620 相同性解析による交差性が予測される生物種
Full Name chromosome 16 open reading frame 62
Observed molecular weight98 kDa
GenBank accession numberBC058845
Gene symbol C16orf62
Gene ID (NCBI) 57020
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VPS35L, also known as EC97, contains 5 N-glycosylation sites, 3 N-myristoylation sites, and numerous phosphorylation sites. Northern blot analysis detected a 3.4-kb EC97 transcript in all human tissues tested except adipose. RT-PCR analysis showed that EC97 expression was upregulated in 60% of human esophageal squamous cell carcinoma (ESCC) tissues and downregulated in 35% of ESCC tissues tested compared with controls. VPS35L is Involved in Golgi to plasma membrane transport and endocytic recycling.

Protocols

Product Specific Protocols
WB protocol for VPS35L antibody 26923-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols