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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C16orf75 Polyclonal antibody (30529-1-AP)

    WB analysis using 30529-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30529-1-AP (C16orf75 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using C16orf75 Polyclonal antibody (30529-1-AP)

    IF Staining of U-251 using 30529-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using C16orf75 antibody (30529-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

C16orf75 Polyclonal antibody

C16orf75 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30529-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

RMI2, RecQ-mediated genome instability protein 2, hRMI2, BLM-associated protein of 18 kDa, BLAP18

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHT-1080 cells, HeLa cells, MCF-7 cells, U-251 cells
Positive IF/ICC detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30529-1-AP targets C16orf75 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C16orf75 fusion protein Ag32880 相同性解析による交差性が予測される生物種
Full Name chromosome 16 open reading frame 75
Observed molecular weight 19 kDa
GenBank accession numberBC031016
Gene Symbol C16orf75
Gene ID (NCBI) 116028
RRIDAB_3086351
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96E14
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C16orf75 is also named as BLM-associated protein of 18 kDa (BLAP18), RMI2, hRMI2 and RecQ-mediated genome instability protein 2. C16orf75 is essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates. It is required to regulate sister chromatid segregation and to limit DNA crossover. And RMI2 is essential for the stability, localization, and function of BLM, TOP3A, and complexes containing BLM. In the RMI complex, it is required to target BLM to chromatin and stress-induced nuclear foci and mitotic phosphorylation of BLM (PMID: 18923082, PMID: 18923083, PMID: 27977684).

Protocols

Product Specific Protocols
WB protocol for C16orf75 antibody 30529-1-APDownload protocol
IF protocol for C16orf75 antibody 30529-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols