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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C16orf84 Polyclonal antibody

C16orf84 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 23337-1-AP

Synonyms

UPF0432, NCS2, Cytosolic thiouridylase subunit 2, Cytoplasmic tRNA 2-thiolation protein 2, CTU2

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using C16orf84 Polyclonal antibody (23337-1-AP)

    WB analysis of HepG2 using 23337-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 23337-1-AP (C16orf84 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using C16orf84 Polyclonal antibody (23337-1-AP)

    WB analysis of mouse liver using 23337-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 23337-1-AP (C16orf84 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using C16orf84 Polyclonal antibody (23337-1-AP)

    WB analysis of Raji using 23337-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 23337-1-AP (C16orf84 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using C16orf84 Polyclonal antibody (23337-1-AP)

    WB analysis of mouse spleen using 23337-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 23337-1-AP (C16orf84 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using C16orf84 Polyclonal antibody (23337-1-AP)

    IP experiment of HepG2 using 23337-1-AP

    IP result of anti-C16orf84 (IP:23337-1-AP, 4ug; Detection:23337-1-AP 1:500) with HepG2 cells lysate 3600ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using C16orf84 Polyclonal antibody (23337-1-AP)

    IHC staining of human testis using 23337-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23337-1-AP (C16orf84 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using C16orf84 Polyclonal antibody (23337-1-AP)

    IHC staining of human kidney using 23337-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23337-1-AP (C16orf84 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using C16orf84 Polyclonal antibody (23337-1-AP)

    IHC staining of human kidney using 23337-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23337-1-AP (C16orf84 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using C16orf84 Polyclonal antibody (23337-1-AP)

    IHC staining of human testis using 23337-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23337-1-AP (C16orf84 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using C16orf84 Polyclonal antibody (23337-1-AP)

    IF Staining of HEK-293 using 23337-1-AP

    Immunofluorescent analysis of HEK-293 cells using 23337-1-AP (C16orf84 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells, mouse liver tissue, mouse spleen tissue, Raji cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23337-1-AP targets C16orf84 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C16orf84 fusion protein Ag18428 相同性解析による交差性が予測される生物種
Full Name chromosome 16 open reading frame 84
Calculated molecular weight 515 aa, 56 kDa
Observed molecular weight 65-68 kDa
GenBank accession numberBC125269
Gene symbol C16orf84
Gene ID (NCBI) 348180
RRIDAB_2879257
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cytoplasmic tRNA 2-thiolation protein 2 (CUT2), also named as C16orf84 or NCS2, is a 515 amino acid protein, which belongs to the CUT2 family. C16orf84 localizes in the cytoplasm and Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). C16orf84 may act by forming a heterodimer with CTU1/ATPBD3 that ligates sulfur from thio-carboxylated URM1 onto the uridine of tRNAs at wobble position.

Protocols

Product Specific Protocols
WB protocol for C16orf84 antibody 23337-1-APDownload protocol
IHC protocol for C16orf84 antibody 23337-1-APDownload protocol
IF protocol for C16orf84 antibody 23337-1-APDownload protocol
IP protocol for C16orf84 antibody 23337-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols