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Species Reactivity

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  • Featured Product
  • KD/KO Validated

C17orf28 Polyclonal antibody

C17orf28 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 21174-1-AP

Synonyms

Protein hid-1 homolog, Protein HID1, HID1 domain-containing protein, HID1, Down-regulated in multiple cancers 1

Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using C17orf28 Polyclonal antibody (21174-1-AP)

    WB analysis of fetal human brain using 21174-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 21174-1-AP (C17orf28 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using C17orf28 Polyclonal antibody (21174-1-AP)

    WB analysis of human brain using 21174-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 21174-1-AP (C17orf28 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using C17orf28 Polyclonal antibody (21174-1-AP)

    IP experiment of mouse brain using 21174-1-AP

    IP result of anti-C17orf28 (IP:21174-1-AP, 3ug; Detection:21174-1-AP 1:500) with mouse brain tissue lysate 10000ug.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using C17orf28 Polyclonal antibody (21174-1-AP)

    IHC staining of human stomach cancer using 21174-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21174-1-AP (C17orf28 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using C17orf28 Polyclonal antibody (21174-1-AP)

    IF Staining of HepG2 using 21174-1-AP

    Immunofluorescent analysis of HepG2 cells, using C17orf28 antibody 21174-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using C17orf28 Polyclonal antibody (21174-1-AP)

    IF Staining of HeLa using 21174-1-AP

    Immunofluorescent analysis of Hela cells, using C17orf28 antibody 21174-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

Positive WB detected infetal human brain tissue, human brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21174-1-AP targets C17orf28 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C17orf28 fusion protein Ag15427 相同性解析による交差性が予測される生物種
Full Name chromosome 17 open reading frame 28
Calculated molecular weight 788 aa, 89 kDa
Observed molecular weight 60 kDa, 89 kDa
GenBank accession numberBC035372
Gene symbol C17orf28
Gene ID (NCBI) 283987
RRIDAB_10733124
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for C17orf28 antibody 21174-1-APDownload protocol
IHC protocol for C17orf28 antibody 21174-1-APDownload protocol
IF protocol for C17orf28 antibody 21174-1-APDownload protocol
IP protocol for C17orf28 antibody 21174-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Elife

HID-1 is required for homotypic fusion of immature secretory granules during maturation.

Authors - Wen Du
  • KO Validated
View Article
ratWB

Biophys Rep

PMID: 30596141

Authors - Min Li
View Article