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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C17orf46 Polyclonal antibody

C17orf46 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24629-1-AP

Synonyms

C17orf46

Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using C17orf46 Polyclonal antibody (24629-1-AP)

    WB analysis of L02 using 24629-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 24629-1-AP (C17orf46 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using C17orf46 Polyclonal antibody (24629-1-AP)

    IHC staining of human testis using 24629-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 24629-1-AP (C17orf46 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using C17orf46 Polyclonal antibody (24629-1-AP)

    IHC staining of human testis using 24629-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 24629-1-AP (C17orf46 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using C17orf46 Polyclonal antibody (24629-1-AP)

    IHC staining of human brain using 24629-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain slide using 24629-1-AP (C17orf46 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using C17orf46 Polyclonal antibody (24629-1-AP)

    IHC staining of human brain using 24629-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain slide using 24629-1-AP (C17orf46 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inL02 cells
Positive IHC detected inhuman testis tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24629-1-AP is C17orf46 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C17orf46 fusion protein Ag18239 相同性解析による交差性が予測される生物種
Full Name chromosome 17 open reading frame 46
Calculated molecular weight 384 aa, 42 kDa
Observed molecular weight 42-52 kDa
GenBank accession numberBC028124
Gene symbol C17orf46
Gene ID (NCBI) 124783
RRIDAB_2879646
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C17orf46, also named as SPATA32 and TEX34, mainly expressed in Testis. It interacts with syntaxin-1 and ACTB. It's abou 42-52 kDa in our detection.

Protocols

Product Specific Protocols
WB protocol for C17orf46 antibody 24629-1-APDownload protocol
IHC protocol for C17orf46 antibody 24629-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols