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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Tim21 Polyclonal antibody

Tim21 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16641-1-AP

Synonyms

C18orf55, HSPC154, Mitochondrial import inner membrane translocase subunit Tim21, TIM21, TIMM21

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of human liver tissue using Tim21 Polyclonal antibody (16641-1-AP)

    IHC staining of human liver using 16641-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16641-1-AP (Tim21 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using Tim21 Polyclonal antibody (16641-1-AP)

    IHC staining of human liver using 16641-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16641-1-AP (Tim21 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Tim21 Polyclonal antibody (16641-1-AP)

    IHC staining of mouse heart using 16641-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16641-1-AP (Tim21 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Tim21 Polyclonal antibody (16641-1-AP)

    IHC staining of mouse heart using 16641-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16641-1-AP (Tim21 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inhuman liver tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16641-1-AP targets Tim21 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Tim21 fusion protein Ag10002 相同性解析による交差性が予測される生物種
Full Name chromosome 18 open reading frame 55
Calculated molecular weight 28 kDa
GenBank accession numberBC000892
Gene symbol Tim21
Gene ID (NCBI) 29090
RRIDAB_2062082
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for Tim21 antibody 16641-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Mitochondrial protein sorting as a therapeutic target for ATP synthase disorders.

Authors - Raeka S Aiyar
View Article
humanWB, IF

Int J Gynecol Cancer

Establishment of paclitaxel-resistant cell line and the underlying mechanism on drug resistance.

Authors - Jingjing Zhang
View Article