• Featured Product
  • KD/KO Validated

C19orf21 Polyclonal antibody

C19orf21 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 26338-1-AP

Synonyms

C19orf21, MISP, Mitotic interactor and substrate of PLK1


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Tested Applications

Positive WB detected inMCF-7 cells, HepG2 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26338-1-AP targets C19orf21 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C19orf21 fusion protein Ag23712 相同性解析による交差性が予測される生物種
Full Name chromosome 19 open reading frame 21
Observed molecular weight 75 kDa
GenBank accession numberBC052236
Gene symbol C19orf21
Gene ID (NCBI) 126353
RRIDAB_2880482
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C19orf21, also named as MISP, plays a role in mitotic spindle orientation and mitotic progression. It may be required for directed cell migration and centrosome orientation. C19orf21 regulates the distribution of dynactin at the cell cortex in a PLK1-dependent manner.

Protocols

Product Specific Protocols
WB protocol for C19orf21 antibody 26338-1-APDownload protocol
IF protocol for C19orf21 antibody 26338-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Oncol

Up-Regulated MISP Is Associated With Poor Prognosis and Immune Infiltration in Pancreatic Ductal Adenocarcinoma.

Authors - Xinyang Huang
humanWB

Cell Death Discov

Long noncoding RNA LINC00284 facilitates cell proliferation in papillary thyroid cancer via impairing miR-3127-5p targeted E2F7 suppression.

Authors - Bin Zhou
mouseWB,IF

J Vet Med Sci

Mitotic spindle positioning protein (MISP) deficiency exacerbates dextran sulfate sodium (DSS)-induced colitis in mice

Authors - Koki Hiura
  • KO Validated
humanWB,IF,IP

Int J Mol Sci

Mitotic Spindle Positioning (MISP) Facilitates Colorectal Cancer Progression by Forming a Complex with Opa Interacting Protein 5 (OIP5) and Activating the JAK2-STAT3 Signaling Pathway

Authors - Koki Hiura
  • KD Validated
humanWB

Front Pharmacol

Protein and metabolic profiles of tyrosine kinase inhibitors co-resistant liver cancer cells

Authors - Zengbin Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

anwesh (Verified Customer) (07-29-2019)

The protein was found at the right size and no additional background bands were observed around the size range. Perfect!

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: 293T, HaCaT, U2OS
C19orf21 Antibody Western Blot, validation (1:1000 dilution) in 293T, HaCaT, U2OS (Cat no:26338-1-AP)